Wabuke-Bunoti M A, Fan D P, Braciale T J
J Immunol. 1981 Sep;127(3):1122-5.
Purified hemagglutinin of influenza virus, subtype A/Jap/305/57 (H2/N2) was cleaved with CNBr, and the resulting fragments were capable of stimulating H-2 restricted cytolytic T lymphocytes in vitro from spleen cells of mice immunized previously with A/Jap/305/57. When the peptides were separated by high-performance liquid chromatography, stimulatory activity was confined primarily to a series of peptides contained within a limited chromatographic range. The majority of the lytic activity was specific for cells infected with A/Jap virus, although a small amount of cross-reactive killing of A/PR/8/34-(H0/N1) infected cells could not be excluded. These results demonstrated that H-2-restricted influenza viral-specific CTL could be generated by fragments of a complex polypeptide antigen, the influenza hemagglutinin, and offer the possibility of analyzing specific antigenic determinants recognized by cytolytic T cells.
用溴化氰裂解A/Jap/305/57（H2/N2）亚型流感病毒的纯化血凝素，所得片段能够在体外刺激先前用A/Jap/305/57免疫的小鼠脾细胞产生H-2限制性细胞毒性T淋巴细胞。当通过高效液相色谱法分离这些肽时，刺激活性主要局限于有限色谱范围内的一系列肽。大部分裂解活性对感染A/Jap病毒的细胞具有特异性，尽管不能排除对感染A/PR/8/34-（H0/N1）细胞的少量交叉反应杀伤。这些结果表明，H-2限制性流感病毒特异性CTL可由复杂多肽抗原流感血凝素的片段产生，并为分析细胞毒性T细胞识别的特异性抗原决定簇提供了可能性。
