A computer-assisted spectrophotometric enzyme assay. A method for the calculation of Km and V from a single reaction progress curve by the integrated form of the Lineweaver-Burke plot with particular reference to beta-lactamase.
作者信息
Fukagawa Y
出版信息
Biochem J. 1980 Jan 1;185(1):186-8. doi: 10.1042/bj1850186.
Abstract
摘要
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1
A computer-assisted spectrophotometric enzyme assay. A method for the calculation of Km and V from a single reaction progress curve by the integrated form of the Lineweaver-Burke plot with particular reference to beta-lactamase.一种计算机辅助分光光度酶法测定。一种通过Lineweaver-Burke图的积分形式从单条反应进程曲线计算Km和V的方法，特别涉及β-内酰胺酶。
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引用本文的文献
1
Inhibition of beta-lactamase of Bacillus licheniformis 749/C by compound PS-5, a new beta-lactam antibiotic.新型β-内酰胺抗生素化合物PS-5对地衣芽孢杆菌749/Cβ-内酰胺酶的抑制作用
Biochem J. 1980 Jan 1;185(1):177-85. doi: 10.1042/bj1850177.
本文引用的文献
1
Inhibition of beta-lactamase of Bacillus licheniformis 749/C by compound PS-5, a new beta-lactam antibiotic.新型β-内酰胺抗生素化合物PS-5对地衣芽孢杆菌749/Cβ-内酰胺酶的抑制作用
Biochem J. 1980 Jan 1;185(1):177-85. doi: 10.1042/bj1850177.
2
A spectrophotometric assay of beta-lactamase action on penicillins.一种关于β-内酰胺酶对青霉素作用的分光光度测定法。
Biochem J. 1974 Jun;139(3):789-90. doi: 10.1042/bj1390789.
3
A simple method for calculating Km and V from a single enzyme reaction progress curve.一种从单一酶促反应进程曲线计算Km和V的简单方法。
Biochim Biophys Acta. 1977 Jan 11;480(1):1-13. doi: 10.1016/0005-2744(77)90315-1.
4
A direct spectrophotometric assay and determination of Michaelis constants for the beta-lactamase reaction.一种用于β-内酰胺酶反应的直接分光光度测定法及米氏常数的测定。
Anal Biochem. 1975 Jan;63(1):17-26. doi: 10.1016/0003-2697(75)90185-2.
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