Ellner J J, Olds G R, Kamel R, Osman G S, el-Kholy A, Mahmoud A A
J Immunol. 1980 Jul;125(1):308-12.
Splenic suppressor cell activity was evaluated in 10 patients with advanced hepatosplenic Schistosomiasis mansoni undergoing elective splenectomy. We used cell mixing experiments to assess the effect of mitomycin-C-treated spleen cells on antigen and mitogen-induced 3H-thymidine incorporation of responder cells. The suppressor to responder ratio was 1.0. Spleen cells from 7 of 10 patients caused at least 20% suppression of phytohemagglutinin-induced 3H-thymidine incorporation of one or more populations of responder cells (spleen cells, autologous and allogeneic peripheral blood mononuclear cells)Responses of peripheral blood mononuclear cells to streptokinase-streptodornase and schistosome soluble egg and worm antigen preparations also were inhibited by co-cultured spleen cells. An inverse correlation was apparent between the spleen cell response to PHA and the suppressor activity of that spleen cell population (r = -0.74, p less than 0.05). Cell purification procedures showed that the active suppressor splenic cell was non-adherent and rosetted neuraminidase-treated sheep erythrocytes. This splenic suppressor T lymphocyte may modulate splenic and peripheral blood lymphocyte responses in patients with hepatosplenic schistosomiasis.
对10例晚期曼氏血吸虫病肝脾肿大且接受择期脾切除术的患者进行了脾抑制细胞活性评估。我们采用细胞混合实验来评估经丝裂霉素-C处理的脾细胞对抗原和丝裂原诱导的应答细胞3H-胸腺嘧啶核苷掺入的影响。抑制细胞与应答细胞的比例为1.0。10例患者中有7例的脾细胞至少使一种或多种应答细胞群体（脾细胞、自体和异体外周血单个核细胞）的植物血凝素诱导的3H-胸腺嘧啶核苷掺入受到20%以上的抑制。共培养的脾细胞也抑制了外周血单个核细胞对链激酶-链道酶以及血吸虫可溶性虫卵和虫体抗原制剂的反应。脾细胞对PHA的反应与该脾细胞群体的抑制活性之间存在明显的负相关（r = -0.74，p < 0.05）。细胞纯化程序显示，具有活性的抑制性脾细胞不黏附，且能与经神经氨酸酶处理的绵羊红细胞形成花环。这种脾抑制性T淋巴细胞可能调节肝脾型血吸虫病患者的脾和外周血淋巴细胞反应。
