de Martinville B, Wyman A R, White R, Francke U
Am J Hum Genet. 1982 Mar;34(2):216-26.
A locus responsible for a restriction fragment length polymorphism (RFLP) has been identified by hybridization of Eco RI fragments to the random human DNA sequence in recombinant plasmid pAW101. We have examined DNA extracted from 20 human X Chinese hamster somatic cell hybrids for the presence of sequences homologous to the human insert in pAW101. The hybrids were derived from six different human donors, five of whom were heterozygous, producing two bands on Southern transfers. The presence of homologous sequences in the hybrids correlated exclusively with the presence of human chromosome 14. Three hybrids contained chromosome 14 in a frequency of greater than one per cell and were positive for two alleles. Two hybrids contained only the distal half of the long arm of 14 as part of a translocation and were still positive. These results assign the first highly polymorphic random RFPL locus (D14S1) to region q21 leads to qter of chromosome 14.
通过将Eco RI片段与重组质粒pAW101中的随机人类DNA序列杂交，已鉴定出一个与限制性片段长度多态性（RFLP）相关的基因座。我们检测了从20个人类X中国仓鼠体细胞杂种中提取的DNA，以确定是否存在与pAW101中人类插入片段同源的序列。这些杂种来自六个不同的人类供体，其中五个是杂合子，在Southern印迹转移上产生两条带。杂种中同源序列的存在仅与人类14号染色体的存在相关。三个杂种细胞中14号染色体的频率大于每细胞一条，并且两个等位基因均呈阳性。两个杂种仅含有14号染色体长臂的远端一半作为易位的一部分，但仍呈阳性。这些结果将第一个高度多态性的随机RFPL基因座（D14S1）定位于14号染色体的q21至qter区域。
