Leszczynski D E, Cleveland J C, Kummerow F A
Clin Cardiol. 1980 Aug;3(4):252-9. doi: 10.1002/clc.4960030206.
Preoperative plasma samples from coronary artery bypass surgery patients were analyzed for relative levels of high-density lipoproteins (HDL) and low-density lipoproteins (LDL) in order to assess the HDL:LDL ratio as an indicator of risk for coronary heart disease. Fasting blood samples were drawn from bypass patients prior to surgery, and bypass patient mean plasma lipid data were compared with values from three other groups: randomly selected, newly admitted hospitalized patients; acutely ill student outpatients; and healthy hospital employees. Sudan Black B prestained plasma samples were electrophoresed on polyacrylamide gels, and HDL:LDL ratios were determined by densitometric gel scan and automatic peak integration. Of the four study groups, the bypass patient group had the lowest mean HDL:LDL ratio; the low ratios were due particularly to reduced levels of HDL2. Chemical analyses indicated that the bypass patient group also had lower mean HDL-cholesterol and higher mean total cholesterol and triglyceride levels than the other groups. When group age and sex differences were taken into account, the bypass group, especially males, also had low ratios. The results also strongly indicated that stresses involved with illness and hospitalization unrelated to clinical coronary heart disease (CHD) can adversely affect lipoprotein patterns.
对冠状动脉搭桥手术患者的术前血浆样本进行分析，以检测高密度脂蛋白（HDL）和低密度脂蛋白（LDL）的相对水平，从而评估HDL与LDL的比值作为冠心病风险指标的情况。在手术前采集搭桥患者的空腹血样，并将搭桥患者的平均血浆脂质数据与其他三组数据进行比较：随机选取的新入院住院患者；急性病学生门诊患者；以及健康的医院员工。用苏丹黑B预染的血浆样本在聚丙烯酰胺凝胶上进行电泳，并通过光密度凝胶扫描和自动峰积分法测定HDL与LDL的比值。在四个研究组中，搭桥患者组的平均HDL与LDL比值最低；低比值尤其归因于HDL2水平的降低。化学分析表明，搭桥患者组的平均HDL胆固醇水平也低于其他组，而平均总胆固醇和甘油三酯水平则高于其他组。当考虑到组间年龄和性别差异时，搭桥组，尤其是男性，其比值也较低。结果还强烈表明，与临床冠心病（CHD）无关的疾病和住院相关应激会对脂蛋白模式产生不利影响。
