重复序列在两栖动物贝氏林蛙（Rana berlandieri）中的散布情况。
Interspersion of repetitive with repetitive sequences in an amphibian, Rana berlandieri.
作者信息
Graham D E, Schanke K E
出版信息
Nucleic Acids Res. 1980 Sep 11;8(17):3875-94. doi: 10.1093/nar/8.17.3875.
Abstract
When conventional genome arrangement analyses performed on R. berlandieri DNA at a normal (60 degrees C) and a high (75 degrees C) reassociation temperature were compared, an additional interspersion pattern was detected which indicates that different classes of repetitive sequences are closely interspersed with each other. Our results further suggest that the genomic abundance of purified (or cloned) repetitive sequences can be accurately determined by solution hybridization with genomic DNA only when the reassociation is performed at a relatively high temperature (Tm - 10 degrees C).
摘要
当对伯氏根霉DNA在正常（60摄氏度）和高（75摄氏度）复性温度下进行常规基因组排列分析并比较时，检测到一种额外的散布模式，这表明不同类别的重复序列彼此紧密散布。我们的结果进一步表明，只有当在相对较高温度（Tm - 10摄氏度）下进行复性时，通过与基因组DNA进行溶液杂交才能准确测定纯化（或克隆）重复序列的基因组丰度。
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