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[人扁桃体淋巴细胞的分析与分级分离]

[Analysis and fractionation of human tonsillar lymphocytes].

作者信息

Csuka I, Vincze I, Antoni F

出版信息

Tsitologiia. 1980 Nov;22(11):1351-6.

PMID:6969480
Abstract

Among the human tonsillar cells, 28.0 +/- 3.1 and 45.14 +/- 2.16% were found to be E and EAC rosette forming cells, resp. In the course of the separation procedure with nylon cotton, in the authors' modification, the cells failing to form rosettes were almost entirely adhering irreversibly to the nylon wool. The 3H-thymidine incorporation in these cells was very intensive, and we supposed them to be T and B lymphoblasts. On the basis of E and EAC rosette formation, the percentage of T and B lymphocytes in human tonsils--as reported by various authors--is different. It may be supposed that methods employed by these authors did not demonstrate the equal extent of transformed forms of lymphocytes, i.e. lymphoblasts, whose amount may be influenced by the functional condition of tonsils.

摘要

在人扁桃体细胞中,发现分别有28.0±3.1%和45.14±2.16%的细胞形成E和EAC玫瑰花结。在作者改进的用尼龙棉进行的分离过程中,未能形成玫瑰花结的细胞几乎完全不可逆地附着于尼龙棉。这些细胞中3H-胸腺嘧啶核苷的掺入非常强烈,我们推测它们是T和B淋巴母细胞。根据E和EAC玫瑰花结的形成情况,不同作者报道的人扁桃体中T和B淋巴细胞的百分比有所不同。可以推测,这些作者采用的方法没有显示出淋巴细胞转化形式(即淋巴母细胞)的同等程度,其数量可能受扁桃体功能状态的影响。

相似文献

1
[Analysis and fractionation of human tonsillar lymphocytes].[人扁桃体淋巴细胞的分析与分级分离]
Tsitologiia. 1980 Nov;22(11):1351-6.
2
Immunological function of tonsils in atopic and nonatopic children, with special reference to E- and EAC-binding lymphocytes.特应性和非特应性儿童扁桃体的免疫功能,特别涉及E和EAC结合淋巴细胞。
Allerg Immunol (Leipz). 1978;24(4):287-93.
3
[Immunologic function of the tonsils in relation to immune competent T and B cells in children].[儿童扁桃体与免疫活性T细胞和B细胞相关的免疫功能]
Padiatr Padol. 1980;15(1):11-8.
4
PHA stimulation of separated human lymphocyte populations.人淋巴细胞分离群体的PHA刺激
Clin Exp Immunol. 1975 Sep;21(3):456-67.
5
Co-operation between T and B lymphocytes from human tonsils in the response to mitogens and antigens.人扁桃体中T淋巴细胞和B淋巴细胞在对有丝分裂原和抗原反应中的合作。
Clin Exp Immunol. 1977 May;28(2):332-40.
6
[Immunomorphologic characteristics of the tonsils and their lymph nodes in human fetuses].[人类胎儿扁桃体及其淋巴结的免疫形态学特征]
Vestn Otorinolaringol. 1989 Mar-Apr(2):55-8.
7
Mouse red cell rosette formation by subpopulations of human lymphocytes.人淋巴细胞亚群形成小鼠红细胞玫瑰花结
Clin Exp Immunol. 1977 Jan;27(1):172-7.
8
Electrophoretic mobility of peripheral non-B human Fc gamma-receptors bearing lymphocytes.携带外周非B人类Fcγ受体的淋巴细胞的电泳迁移率。
Biomedicine. 1980 Feb;32(1):31-41.
9
[Demonstration of T-lymphocyte differentiation in the tonsil by determination of surface markers with monoclonal antibodies and rosette technics].
Allerg Immunol (Leipz). 1984;30(3):169-74.
10
Rosette formation with mouse erythrocytes. IV. T, B and third population cells in human tonsils.与小鼠红细胞形成玫瑰花结。IV. 人类扁桃体中的T细胞、B细胞和第三类细胞。
Clin Exp Immunol. 1977 May;28(2):347-51.

引用本文的文献

1
Topo-optical reactions and polarization optical analysis of human lymphocytes.人类淋巴细胞的拓扑光学反应与偏振光学分析
Histochemistry. 1983;79(2):269-79. doi: 10.1007/BF00489789.