巨噬细胞弹性蛋白酶介导的有限蛋白水解作用可使人类α1-蛋白酶抑制剂失活。
Limited proteolysis by macrophage elastase inactivates human alpha 1-proteinase inhibitor.
作者信息
Banda M J, Clark E J, Werb Z
出版信息
J Exp Med. 1980 Dec 1;152(6):1563-70. doi: 10.1084/jem.152.6.1563.
Abstract
Inflammatory mouse peritoneal macrophages secrete a metalloproteinase that is not inhibited by alpha 1-proteinase inhibitor. This proteinase, macrophage elastase, recognizes alpha 1-proteinase inhibitor with macrophage elastase does not involve a stable proteinase-inhibitor complex and results in the proteolytic removal of a peptide of apparent molecular weight 4,000-5,000 from the inhibitor. After degradation by macrophage elastase, alpha 1-proteinase inhibitor is no longer able to inhibit human granulocyte elastase, a serine proteinase implicated in the pathogenesis of emphysema. Macrophage elastase apparently does not degrade human granulocyte elastase-alpha 1-proteinase inhibitor complexes or release active granulocyte elastase from these complexes. The ability of macrophage elastase to degrade alpha 1-proteinase inhibitor is inhibited by EDTA and alpha 2-macroglobulin.
摘要
炎性小鼠腹腔巨噬细胞分泌一种金属蛋白酶，该酶不受α1-蛋白酶抑制剂的抑制。这种蛋白酶即巨噬细胞弹性蛋白酶，它识别α1-蛋白酶抑制剂的方式并不涉及稳定的蛋白酶-抑制剂复合物，而是导致从该抑制剂上蛋白水解去除一个表观分子量为4000-5000的肽段。经巨噬细胞弹性蛋白酶降解后，α1-蛋白酶抑制剂不再能够抑制人粒细胞弹性蛋白酶，后者是一种与肺气肿发病机制有关的丝氨酸蛋白酶。巨噬细胞弹性蛋白酶显然不会降解人粒细胞弹性蛋白酶-α1-蛋白酶抑制剂复合物，也不会从这些复合物中释放出活性粒细胞弹性蛋白酶。EDTA和α2-巨球蛋白可抑制巨噬细胞弹性蛋白酶降解α1-蛋白酶抑制剂的能力。
相似文献
1
Limited proteolysis by macrophage elastase inactivates human alpha 1-proteinase inhibitor.巨噬细胞弹性蛋白酶介导的有限蛋白水解作用可使人类α1-蛋白酶抑制剂失活。
J Exp Med. 1980 Dec 1;152(6):1563-70. doi: 10.1084/jem.152.6.1563.
2
Mouse macrophage elastase. Purification and characterization as a metalloproteinase.小鼠巨噬细胞弹性蛋白酶。作为金属蛋白酶的纯化及特性鉴定。
Biochem J. 1981 Feb 1;193(2):589-605. doi: 10.1042/bj1930589.
3
Alpha 1-proteinase inhibitor is a neutrophil chemoattractant after proteolytic inactivation by macrophage elastase.α1-蛋白酶抑制剂在被巨噬细胞弹性蛋白酶进行蛋白水解失活后是一种中性粒细胞趋化剂。
J Biol Chem. 1988 Mar 25;263(9):4481-4.
4
Partial purification and characterization of mouse peritoneal exudative macrophage elastase.小鼠腹腔渗出巨噬细胞弹性蛋白酶的部分纯化及特性研究
Biochim Biophys Acta. 1980 Mar 14;612(1):233-44. doi: 10.1016/0005-2744(80)90297-1.
5
Interaction of mouse macrophage elastase with native and oxidized human alpha 1-proteinase inhibitor.小鼠巨噬细胞弹性蛋白酶与天然及氧化型人α1-蛋白酶抑制剂的相互作用
J Clin Invest. 1987 May;79(5):1314-7. doi: 10.1172/JCI112955.
6
Granulocyte elastase-mediated proteolysis of alpha 1-antitrypsin in cystic fibrosis bronchopulmonary secretions.囊性纤维化支气管肺分泌物中粒细胞弹性蛋白酶介导的α1抗胰蛋白酶蛋白水解作用
Pediatr Pulmonol. 1989;7(1):12-7. doi: 10.1002/ppul.1950070105.
7
Oxidized alpha 1-proteinase inhibitor: a fast-acting inhibitor of human pancreatic elastase.氧化α1-蛋白酶抑制剂：一种人胰腺弹性蛋白酶的快速作用抑制剂。
Biochim Biophys Acta. 1992 Jan 9;1118(2):174-8. doi: 10.1016/0167-4838(92)90147-6.
8
Pathobiochemical significance of granulocyte elastase complexed with proteinase inhibitors: effect on glycosaminoglycan metabolism in cultured synovial cells.与蛋白酶抑制剂复合的粒细胞弹性蛋白酶的病理生化意义：对培养滑膜细胞中糖胺聚糖代谢的影响。
J Clin Chem Clin Biochem. 1987 Mar;25(3):151-60. doi: 10.1515/cclm.1987.25.3.151.
9
Uptake of immunoreactive leukocyte elastase-inhibitor complexes in macrophage-like cells in abscesses.脓肿中巨噬细胞样细胞对免疫反应性白细胞弹性蛋白酶抑制剂复合物的摄取。
Eur Surg Res. 1991;23(3-4):256-60. doi: 10.1159/000129161.
10
Regulation of alpha 1 proteinase inhibitor function by rabbit alveolar macrophages. Evidence for proteolytic rather than oxidative inactivation.兔肺泡巨噬细胞对α1蛋白酶抑制剂功能的调节。蛋白水解而非氧化失活的证据。
J Clin Invest. 1985 Jun;75(6):1758-62. doi: 10.1172/JCI111887.
引用本文的文献
1
The Role of Serine Proteases and Antiproteases in the Cystic Fibrosis Lung.丝氨酸蛋白酶和抗蛋白酶在囊性纤维化肺部中的作用
Mediators Inflamm. 2015;2015:293053. doi: 10.1155/2015/293053. Epub 2015 Jun 21.
2
Age-related macular degeneration and changes in the extracellular matrix.年龄相关性黄斑变性与细胞外基质的变化
Med Sci Monit. 2014 Jun 18;20:1003-16. doi: 10.12659/MSM.889887.
3
Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases.弹性蛋白酶抑制因子经铜绿假单胞菌金属蛋白酶切割后的功能研究。
Biol Chem. 2010 Jun;391(6):705-16. doi: 10.1515/BC.2010.066.
4
Elevated MMP-12 protein levels in induced sputum from patients with COPD.慢性阻塞性肺疾病（COPD）患者诱导痰中基质金属蛋白酶-12（MMP-12）蛋白水平升高。
Thorax. 2006 Mar;61(3):196-201. doi: 10.1136/thx.2005.042432. Epub 2005 Nov 24.
5
6
MMPs and TIMPs--an historical perspective.基质金属蛋白酶与基质金属蛋白酶组织抑制因子——历史视角
Mol Biotechnol. 2002 Sep;22(1):33-49. doi: 10.1385/MB:22:1:033.
7
Matrix metalloproteinases limit functional recovery after spinal cord injury by modulation of early vascular events.基质金属蛋白酶通过调节早期血管事件限制脊髓损伤后的功能恢复。
J Neurosci. 2002 Sep 1;22(17):7526-35. doi: 10.1523/JNEUROSCI.22-17-07526.2002.
8
Potential mechanism of emphysema: alpha 1-proteinase inhibitor recovered from lungs of cigarette smokers contains oxidized methionine and has decreased elastase inhibitory capacity.肺气肿的潜在机制：从吸烟者肺部回收的α1-抗胰蛋白酶含有氧化蛋氨酸，且其抑制弹性蛋白酶的能力降低。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):2041-5. doi: 10.1073/pnas.79.6.2041.
9
Inhibitory capacity of alpha 1 antitrypsin in lung secretions: variability and the effect of drugs.肺分泌物中α1抗胰蛋白酶的抑制能力：变异性及药物的影响。
Thorax. 1984 Jul;39(7):510-6. doi: 10.1136/thx.39.7.510.
10
Pathogenesis of the adult respiratory distress syndrome. Evidence of oxidant activity in bronchoalveolar lavage fluid.成人呼吸窘迫综合征的发病机制。支气管肺泡灌洗液中氧化活性的证据。
J Clin Invest. 1983 Mar;71(3):754-61. doi: 10.1172/jci110823.
本文引用的文献
1
ENZYMATICALLY PRODUCED PULMONARY EMPHYSEMA; A PRELIMINARY REPORT.酶促产生的肺气肿：初步报告。
J Occup Med. 1964 Dec;6:481-4.
2
Degradation of connective tissue matrices by macrophages. I. Proteolysis of elastin, glycoproteins, and collagen by proteinases isolated from macrophages.巨噬细胞对结缔组织基质的降解。I. 从巨噬细胞中分离出的蛋白酶对弹性蛋白、糖蛋白和胶原蛋白的蛋白水解作用。
J Exp Med. 1980 Nov 1;152(5):1340-57. doi: 10.1084/jem.152.5.1340.
3
Comparative studies on native and elastase-inactivated alpha 1-protease inhibitor (alpha 1-antitrypsin).天然型和弹性蛋白酶灭活型α1-蛋白酶抑制剂（α1-抗胰蛋白酶）的比较研究
Biochim Biophys Acta. 1980 Mar 20;628(3):293-302. doi: 10.1016/0304-4165(80)90378-5.
4
On the production of emphysema in Syrian hamsters by aerosol inhalation of papain.关于通过雾化吸入木瓜蛋白酶在叙利亚仓鼠中诱发肺气肿的研究。
Arch Environ Health. 1968 Jan;16(1):59-60. doi: 10.1080/00039896.1968.10665015.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Inhibition by alpha-macroglobulin and other serum proteins.α-巨球蛋白及其他血清蛋白的抑制作用。
Biochem J. 1973 Apr;131(4):823-31. doi: 10.1042/bj1310823.
7
Bacterial inactivation of human serum alpha-1 antitrypsin.人血清α-1抗胰蛋白酶的细菌失活作用
J Lab Clin Med. 1971 May;77(5):777-85.
8
9
Clinical, epidemiologic, and pulmonary function studies in alpha,-antitrypsin-deficient subjects of Pi Z type.Pi Z型α1-抗胰蛋白酶缺乏症患者的临床、流行病学及肺功能研究。
Am Rev Respir Dis. 1976 Nov;114(5):945-53. doi: 10.1164/arrd.1976.114.5.945.
10
Effect of human alpha-antitrypsin in papain-induced emphysema in the hamster.人α-抗胰蛋白酶在木瓜蛋白酶诱导的仓鼠肺气肿中的作用。
Am Rev Respir Dis. 1976 May;113(5):607-12. doi: 10.1164/arrd.1976.113.5.607.
Zotero 插件