Lauria F, Catovsky D
Boll Ist Sieroter Milan. 1980 May 31;59(2):143-6.
Membrane receptors for IgG (Fc gamma) and IgM (Fc mu) were assessed on isolated peripheral blood T-lymphocytes by rosette formation with ox-RBC coated with rabbit IgG or IgM antibodies. The mean percentage of Fc gamma and Fc mu rosettes on freshly separated T-cells was 19% (+/- 6% SD) and 4% (+/- 1% SD), respectively. These values increased significantly to 40% (+/- 9%) for Fc gamma and 34% (+/- 8%) for Fc mu after preincubation of the lymphocytes with Neuraminidase. The proportion of Fc mu, but not of Fc gamma, rosettes increased to 44% (+/- 8 SD) after overnight incubation at 37 degrees C, and less markedly after incubation for 45m at 37 degrees C (29% +/- 7). Fc gamma and Fc mu rosette formation, with or without neuraminidase, was specifically inhibited by human IgG or IgM. These findings suggest (1) that hidden receptors can be demonstrated on T-lymphocytes after Neuraminidase treatment which, in the case of Fc gamma, cannot be revealed by other means, and (2) that the proportion of T gamma lymphocytes in the peripheral blood is higher than generally believed.
通过与包被有兔IgG或IgM抗体的氧化红细胞形成玫瑰花结，对分离出的外周血T淋巴细胞上的IgG（Fcγ）和IgM（Fcμ）膜受体进行评估。新鲜分离的T细胞上Fcγ和Fcμ玫瑰花结的平均百分比分别为19%（±6%标准差）和4%（±1%标准差）。在用神经氨酸酶对淋巴细胞进行预孵育后，Fcγ的这些值显著增加至40%（±9%），Fcμ的这些值显著增加至34%（±8%）。在37℃过夜孵育后，Fcμ（而非Fcγ）玫瑰花结的比例增加至44%（±8标准差），在37℃孵育45分钟后增加不太明显（29%±7）。无论有无神经氨酸酶，Fcγ和Fcμ玫瑰花结的形成均受到人IgG或IgM的特异性抑制。这些发现表明：（1）经神经氨酸酶处理后，T淋巴细胞上可显示出隐藏的受体，就Fcγ而言，其他方法无法揭示这些受体；（2）外周血中Tγ淋巴细胞的比例高于普遍认为的比例。
