Von Eschen K B, Rudbach J A
Infect Immun. 1981 Jan;31(1):327-33. doi: 10.1128/iai.31.1.327-333.1981.
The quantitative and qualitative nature of the antibody responses of euthymic (normal, RML) and athymic (nude) mice injected with dinitrophenylated (DNP) lipopolysaccharide (LPS) was evaluated. Antibody responses to both the haptenic (DNP) and carrier (LPS) determinants were measured. On a quantitative basis, RML and nude mice stimulated with DNP-LPS produced only primary anti-DNP responses, whereas both primary and secondary anti-LPS responses were elicited by this conjugate. The failure of DNP-LPS to trigger secondary anti-DNP responses was not dependent on the amount of DNP-LPS given in the primary or secondary doses and could not be overcome by repeated injections of DNP-LPS. Also, the anti-DNP responses of RML mice injected repeatedly with DNP-LPS were restricted to immunoglobulin M antibodies whereas both immunoglobulin M and G anti-LPS responses were elicited. Nude mice also produced immunoglobulin G antibodies to the LPS determinants. These data showed a dissociation of the anti-hapten and anti-carrier antibody responses and suggested that different immunological signals were functioning in the respective anti-DNP and anti-LPS responses.
对注射二硝基苯基化（DNP）脂多糖（LPS）的正常胸腺（正常、RML）和无胸腺（裸）小鼠的抗体反应的定量和定性性质进行了评估。测量了对半抗原（DNP）和载体（LPS）决定簇的抗体反应。在定量基础上，用DNP-LPS刺激的RML和裸小鼠仅产生初级抗DNP反应，而该偶联物引发了初级和次级抗LPS反应。DNP-LPS未能触发次级抗DNP反应并不取决于初级或次级剂量中给予的DNP-LPS的量，并且不能通过重复注射DNP-LPS来克服。此外，反复注射DNP-LPS的RML小鼠的抗DNP反应仅限于免疫球蛋白M抗体，而引发了免疫球蛋白M和G抗LPS反应。裸小鼠也产生了针对LPS决定簇的免疫球蛋白G抗体。这些数据显示了抗半抗原和抗载体抗体反应的分离，并表明不同的免疫信号在各自的抗DNP和抗LPS反应中起作用。
