用二硝基苯基胸腺素免疫先天性无胸腺（裸）小鼠后的胸腺依赖性抗半抗原反应
Thymic-dependent anti-hapten response in congenitally athymic (nude) mice immunized with DNP-thymosin.
作者信息
Ahmed A, Smith A H, Sell K W, Gershwin M E, Steinberg A D, Thurman G B, Goldstein A L
出版信息
Immunology. 1977 Nov;33(5):757-65.
Immunization of congenitally athymic (nu/nu) and adult thymectomized, irradiated bone marrow, reconstituted (TxBm) mice with DNP5-thymosin (dinitrophenylated-bovine thymosin fraction 5) was found to elcit IgM and IgG anti-DNP plaque-forming cells in these animals. Further studies indicated that this response was antigen specific and not due to polyclonal activation. Since the hormonal properties of the thymosin were retained following linkage with hapten and DNP-thymosin was immunogenic in CBA/N and CBA/N female X DBA/2 male)F1 male mice, animals previously shown to have an X-linked inability to respond to thymus-independent antigens, it was concluded that DNP-thymosin functions both as a hormone and as a T-dependent antigen in eliciting an immune response in nu/nu and TxBm mice. Additional support for this conclusion was provided by the demonstration that DNP-thymosin could specifically prime for and elicit an anamnestic response in nu/nu mice. These results indicate that further investigation of the immune activities of DNP-thymosin may provide valuable insight in characterizing the maturation of helper T cells and their subsequent interaction with B cells.
研究发现，用二硝基苯基化牛胸腺素5（DNP5 - 胸腺素）对先天性无胸腺（nu/nu）以及成年后胸腺切除、经辐照并骨髓重建的（TxBm）小鼠进行免疫，能在这些动物体内引发IgM和IgG抗DNP空斑形成细胞。进一步研究表明，这种反应具有抗原特异性，并非由多克隆激活所致。由于与半抗原连接后胸腺素的激素特性得以保留，且DNP - 胸腺素在CBA/N小鼠以及CBA/N雌性×DBA/2雄性）F1雄性小鼠（先前已证明这些动物存在X连锁的对胸腺非依赖性抗原无反应能力）中具有免疫原性，因此得出结论：在nu/nu和TxBm小鼠中引发免疫反应时，DNP - 胸腺素既发挥激素作用，又作为T依赖性抗原。DNP - 胸腺素能在nu/nu小鼠中特异性致敏并引发回忆反应，这一证明为该结论提供了额外支持。这些结果表明，对DNP - 胸腺素免疫活性的进一步研究可能为阐明辅助性T细胞的成熟及其随后与B细胞的相互作用提供有价值的见解。
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