体外免疫反应的反馈抑制。II. IgVH限制的抗体依赖性抑制。
Feedback suppression of the immune response in vitro. II. IgVH-restricted antibody-dependent suppression.
作者信息
Zubler R H, Benacerraf B, Germain R N
出版信息
J Exp Med. 1980 Mar 1;151(3):681-94. doi: 10.1084/jem.151.3.681.
Feedback suppression of the primary humoral immune response to sheep erythrocytes (SRBC) in vitro was induced with cell-free supernate material derived from antigen-(SRBC) activated B (sIg+) cells. This soluble products bears Ig determinants and binds to the eliciting antigen (SRBC). The activity of this antibody in suppressing anti-SRBC plaque-forming cell responses is restricted to spleen cell cultures containing B cells sharing VH genes with the B cells producing the suppressive antibody. The anti-hapten (trinitrophenyl) response to derivatized SRBC is not affected by antigen-primed B cells or their products. These data are compatible with suppression being mediated by anti-antigen antibody, either (a) via blockade of different SRBC epitopes recognized by a limited set of B cell clones in each mouse strain, (b) via triggering of an anti-idiotypic response, either antibody or suppressor T cell in nature, restricted to activity in cultures containing B cells sharing VH structures with the original antibody, or (c) via interference by preformed antibody with T cell help directed at idiotype bearing B cells.
用源自抗原（绵羊红细胞）激活的B（表面免疫球蛋白阳性）细胞的无细胞上清液材料，在体外诱导对绵羊红细胞（SRBC）的初次体液免疫应答的反馈抑制。这种可溶性产物带有免疫球蛋白决定簇，并与引发抗原（SRBC）结合。这种抗体在抑制抗SRBC空斑形成细胞应答中的活性，仅限于含有与产生抑制性抗体的B细胞共享重链可变区（VH）基因的B细胞的脾细胞培养物。对衍生化SRBC的抗半抗原（三硝基苯基）应答不受抗原致敏的B细胞或其产物的影响。这些数据与抑制作用由抗抗原抗体介导相一致，其方式为：（a）通过阻断每个小鼠品系中一组有限的B细胞克隆所识别的不同SRBC表位；（b）通过引发抗独特型应答，该应答本质上为抗体或抑制性T细胞，仅限于在含有与原始抗体共享VH结构的B细胞的培养物中发挥活性；或（c）通过预先形成的抗体干扰针对带有独特型的B细胞的T细胞辅助作用。
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