Caceci T, Brook I, Daniel A
J Clin Microbiol. 1981 Mar;13(3):540-7. doi: 10.1128/jcm.13.3.540-547.1981.
Nephelometry, an immunological technique widely used for the quantification of blood proteins, was adapted to provide a quantitative method of detecting Haemophilus influenzae capsular antigen in body fluids. Using specific antiserum directed against H. influenzae capsular antigen, samples of serum, cerebrospinal fluid, urine, and joint fluid from 38 cases of H. influenzae infections were analyzed. The results were compared for reliability to counterimmunoelectrophoresis, a widely used diagnostic tool. The nephelometric technique has the same advantages of speed and specificity as counterimmunoelectrophoresis and provides the clinician and researcher with a quantitative method that is as reliable as the qualitative counterimmunoelectrophoresis procedure. The method allowed directly quantitative readouts on patient specimens, with no necessity for serial dilutions or densitometric readings.
散射比浊法是一种广泛用于定量血液蛋白质的免疫技术，现已被改编用于提供一种检测体液中流感嗜血杆菌荚膜抗原的定量方法。使用针对流感嗜血杆菌荚膜抗原的特异性抗血清，对38例流感嗜血杆菌感染患者的血清、脑脊液、尿液和关节液样本进行了分析。将结果与反向免疫电泳（一种广泛使用的诊断工具）的可靠性进行了比较。散射比浊技术具有与反向免疫电泳相同的速度和特异性优势，并为临床医生和研究人员提供了一种与定性反向免疫电泳程序一样可靠的定量方法。该方法可直接对患者标本进行定量读数，无需进行系列稀释或光密度读数。
