用于细菌抗原检测的葡萄球菌协同凝集试验、乳胶凝集试验和对流免疫电泳的比较。
Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection.
作者信息
Thirumoorthi M C, Dajani A S
出版信息
J Clin Microbiol. 1979 Jan;9(1):28-32. doi: 10.1128/jcm.9.1.28-32.1979.
Abstract
Soluble antigens of Haemophilus influenzae type b, Streptococcus pneumoniae, Neisseria meningitidis, and group B streptococcus were looked for in cerebrospinal fluid, serum, and urine by using the staphylococcal coagglutination test, latex agglutination test, and counterimmunoelectrophoresis. The staphylococcal coaggultination and latex agglutination tests were more sensitive than counterimmunoelectrophoresis in identifying antigens of H. influenzae type b, S. pneumoniae, and N. meningitidis. None of the three tests successfully detected group B streptococcal antigens in body fluids. Nonspecific reactions noted with the staphylococcal coagglutination test could be usually eliminated after premixing test specimens with soluble protein A.
摘要
采用葡萄球菌协同凝集试验、乳胶凝集试验和对流免疫电泳法，在脑脊液、血清和尿液中查找b型流感嗜血杆菌、肺炎链球菌、脑膜炎奈瑟菌和B族链球菌的可溶性抗原。在鉴定b型流感嗜血杆菌、肺炎链球菌和脑膜炎奈瑟菌的抗原方面，葡萄球菌协同凝集试验和乳胶凝集试验比对流免疫电泳更敏感。这三种试验均未成功检测到体液中的B族链球菌抗原。葡萄球菌协同凝集试验中出现的非特异性反应通常可在将检测标本与可溶性蛋白A预混合后消除。
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