小鼠T细胞生长因子的纯化。一种具有辅助活性的淋巴细胞有丝分裂原。
Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.
作者信息
Granelli-Piperno A, Vassalli J D, Reich E
出版信息
J Exp Med. 1981 Aug 1;154(2):422-31. doi: 10.1084/jem.154.2.422.
Abstract
Mouse T cell growth factor was purified from the serum-free conditioned medium of lectin-stimulated spleen cells. A 3,000-fold purification was achieved with a final yield of 12%. The purified protein, with an apparent Mr of 23,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, was active at concentrations of 4 x 10(-11) M, both in the T cell growth factor and T cell replacing factor assays. In addition, purified T cell growth factor alone was mitogenic for spleen cells from both nude and normal mice.
摘要
小鼠T细胞生长因子是从凝集素刺激的脾细胞的无血清条件培养基中纯化得到的。实现了3000倍的纯化，最终产率为12%。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳，纯化蛋白的表观分子量为23000，在T细胞生长因子和T细胞替代因子测定中，浓度为4×10(-11)M时具有活性。此外，单独的纯化T细胞生长因子对裸鼠和正常小鼠的脾细胞均有促有丝分裂作用。
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