Seebacher C, Bässler E
Arch Geschwulstforsch. 1981;51(2):179-86.
We examined the number of T-Lymphocytes in 14 malignant melanoma patients using the sheep erythrocyte rosette test and the PHA stimulation test. These investigations were carried out before the operation under general anesthesia was performed as well as 1, 7, 14, 21, and 28 days after the operation. 16 patients with various large surface skin operations in local anesthesia served as a control group. Among them there were also 5 malignant melanoma cases. The tests preoperatively accomplished yielded normal results (T-lymphocytes 65.7 +/- 2.95%, PHA stimulation 79.01 +/- 1.69%) for all 19 melanoma patients. 24 hours after the operation the average portion of T-lymphocytes decreased in relation to the whole lymphocytes and reached only the level of 53.8 +/- 7.8%. After 7 days it amounted to 57.5 +/- 8.5%, after 14 days to 59.5 +/- 7.8%, after 21 days to 62.3 +/- 5.4% and after 28 days to 62.8 +/- 6.1%. The PHA stimulation was significantly diminished only after 24 hours, but not longer after 7 days. A correlation between the diminution of T-lymphocytes observed 14 and more days after the operation and the duration of the general anesthesia may be suggested in single cases. -- Patients operated under local anesthesia showed after 2 and 24 hours a significant diminution of T-lymphocytes. After 7 days the values had returned to normal levels (66.7 +/- 5.9%).
我们使用绵羊红细胞玫瑰花结试验和PHA刺激试验检测了14例恶性黑色素瘤患者的T淋巴细胞数量。这些检测在全身麻醉手术前以及术后1天、7天、14天、21天和28天进行。16例接受局部麻醉下各种大表面积皮肤手术的患者作为对照组。其中也有5例恶性黑色素瘤病例。术前对所有19例黑色素瘤患者进行的检测结果正常（T淋巴细胞65.7±2.95%，PHA刺激79.01±1.69%）。术后24小时，T淋巴细胞相对于全淋巴细胞的平均比例下降，仅达到53.8±7.8%的水平。7天后为57.5±8.5%，14天后为59.5±7.8%，21天后为62.3±5.4%，28天后为62.8±6.1%。PHA刺激仅在24小时后显著降低，但7天后不再降低。在个别病例中，可能提示术后14天及更长时间观察到的T淋巴细胞减少与全身麻醉时间之间存在相关性。——局部麻醉下手术的患者在术后2小时和24小时显示T淋巴细胞显著减少。7天后数值恢复到正常水平（66.7±5.9%）。
