Kubota M, Mikawa H
Clin Exp Immunol. 1981 May;44(2):315-23.
In phytohaemagglutinin (PHA) skin test-positive individuals, rosette-increasing factor (RIF), which augmented active E and EA rosette formation, appeared in the serum following the PHA skin testing. This factor was detectable 6 hr after the application of PHA and reached a peak at 14 hr. The appearance of the factor was closely related to the delayed cutaneous hypersensitivity. When mononuclear (MN) cells derived from individuals exhibiting delayed cutaneous reaction were further cultured without the addition of PHA, a similar property was found in the supernatants. Fractionation of the cells by E rosetting revealed this factor to be the product of T lymphocytes. Moreover, despite a lower percentage in contaminated T lymphocytes, a higher RIF activity was observed in the supernatants of the nylon-wool-retained population. The production of RIF was completely inhibited by cytochalasin B, but was not affected by colchicine. Experiments utilizing cycloheximide revealed that new protein synthesis was only necessary for early activation steps. RIF is a kind of lymphokine synthesized in the active immune process in vivo. Therefore, to assay RIF in vivo may provide a new method for investigating the cellular immune competence of the given patients.
在植物血凝素（PHA）皮肤试验呈阳性的个体中，增强活性E和EA玫瑰花结形成的玫瑰花结增加因子（RIF）在PHA皮肤试验后出现在血清中。该因子在应用PHA后6小时可检测到，并在14小时达到峰值。该因子的出现与迟发性皮肤超敏反应密切相关。当对表现出迟发性皮肤反应的个体来源的单核（MN）细胞在不添加PHA的情况下进一步培养时，在上清液中发现了类似的特性。通过E玫瑰花结对细胞进行分级分离显示该因子是T淋巴细胞的产物。此外，尽管尼龙毛柱保留群体中污染的T淋巴细胞百分比更低，但在上清液中观察到了更高的RIF活性。RIF的产生完全被细胞松弛素B抑制，但不受秋水仙碱影响。利用放线菌酮的实验表明，新的蛋白质合成仅对早期激活步骤是必需的。RIF是一种在体内活性免疫过程中合成的淋巴因子。因此，在体内检测RIF可能为研究特定患者的细胞免疫能力提供一种新方法。
