腮腺炎病毒在人淋巴样细胞系及外周血淋巴细胞中的复制：对T细胞的偏好。
Mumps virus replication in human lymphoid cell lines and in peripheral blood lymphocytes: preference for T cells.
作者信息
Fleischer B, Kreth H W
出版信息
Infect Immun. 1982 Jan;35(1):25-31. doi: 10.1128/iai.35.1.25-31.1982.
The replication of mumps virus was studied in human continuous lymphoblastoid cell lines (LCLs) with T or B characteristics and in lymphocyte subpopulations derived from peripheral blood. T-LCLs supported effective virus replication as shown by high titers of free and cell-associated virus over 1 to 4 days after infection. By immunofluorescence analysis, the majority of cells were positive for mumps virus antigens. In contrast, the B-cell lines produced low titers of infectious virus, and only a small percentage expressed viral antigens. This resistance of the B-LCLs was found with several mumps virus strains. Cultures of peripheral blood mononuclear cells also supported mumps virus replication. Very high titers of infectious virus (10(8) PFU/ml) were observed in cultures prestimulated with phytohaemagglutinin. Studies with enriched T and B cells point to the activated T lymphocyte as the major virus-producing cell.
在具有T或B特性的人连续淋巴母细胞系（LCL）以及源自外周血的淋巴细胞亚群中研究了腮腺炎病毒的复制情况。T-LCL支持有效的病毒复制，感染后1至4天内游离病毒和细胞相关病毒的高滴度即可证明这一点。通过免疫荧光分析，大多数细胞的腮腺炎病毒抗原呈阳性。相比之下，B细胞系产生的感染性病毒滴度较低，只有一小部分表达病毒抗原。几种腮腺炎病毒株均发现B-LCL具有这种抗性。外周血单个核细胞培养物也支持腮腺炎病毒复制。在用植物血凝素预刺激的培养物中观察到非常高的感染性病毒滴度（10⁸ PFU/ml）。对富集的T细胞和B细胞的研究表明，活化的T淋巴细胞是主要的病毒产生细胞。
Zotero 插件