Chantler J K, Tingle A J
J Gen Virol. 1980 Oct;50(2):317-28. doi: 10.1099/0022-1317-50-2-317.
Human mononuclear cells (lymphocytes and monocytes) from peripheral blood were examined for their ability to support the replication of rubella virus (RV) after infection in vitro. Replication was shown to occur in mixed lymphocytes and to be enhanced by stimulation with phytohaemagglutinin or pokeweek mitogen. A comparison of RV polypeptide synthesis in lymphocytes and RK13 cells showed no major differences in the polypeptides induced by infection. However, cellular translation was inhibited in the lymphocytes facilitating identification of virus polypeptides and eliminating the need for hypertonic labelling conditions used with RK13 cells. RV replication was also shown to occur in sub-populations of T-cells but not in B-cells. However, RV could be rescued from the B and monocyte population by co-cultivation with RK13 cells.
对外周血中的人单核细胞（淋巴细胞和单核细胞）进行检测，以观察其在体外感染后支持风疹病毒（RV）复制的能力。结果显示，风疹病毒能在混合淋巴细胞中复制，且用植物血凝素或商陆有丝分裂原刺激可增强其复制。淋巴细胞与RK13细胞中风疹病毒多肽合成的比较表明，感染诱导产生的多肽没有重大差异。然而，淋巴细胞中的细胞翻译受到抑制，这有利于鉴定病毒多肽，并且无需使用RK13细胞时所采用的高渗标记条件。风疹病毒复制也显示发生在T细胞亚群中，而不在B细胞中。不过，通过与RK13细胞共培养，可从B细胞和单核细胞群体中拯救出风疹病毒。
