Davey M P, Korngold L
Int Arch Allergy Appl Immunol. 1982;67(3):278-83. doi: 10.1159/000233031.
A solid-phase assay for the detection of anti-F(ab')2 antibodies is described. Wells of microtiter plates are coated with F(ab')2, dilutions of sera are added, and IgG bound to the solid phase is detected using peroxidase-labeled Protein A. Anti-F(ab')2 antibodies were found in 61% of sera from patients with rheumatoid arthritis, 77% with subacute bacterial endocarditis , and 34% with systemic lupus erythematosus. Simultaneous analysis of these sera for immune complexes (IC), using modified Clq and monoclonal rheumatoid factor assays, showed that a correlation existed between anti-F(ab')2 antibodies and the levels of IC. Characterization of anti-F(ab')2 antibodies by inhibition and absorption experiments and by immunological and physical means indicated that they were similar to serum proteins described in the 1960s and designated pepsin agglutinators.
本文描述了一种用于检测抗F(ab')2抗体的固相分析方法。微量滴定板的孔用F(ab')2包被，加入血清稀释液，然后使用过氧化物酶标记的蛋白A检测结合到固相上的IgG。在类风湿性关节炎患者的61%血清、亚急性细菌性心内膜炎患者的77%血清和系统性红斑狼疮患者的34%血清中发现了抗F(ab')2抗体。使用改良的Clq和单克隆类风湿因子分析方法对这些血清进行免疫复合物（IC）的同步分析表明，抗F(ab')2抗体与IC水平之间存在相关性。通过抑制和吸收实验以及免疫学和物理方法对抗F(ab')2抗体进行表征，结果表明它们与20世纪60年代描述的血清蛋白相似，被称为胃蛋白酶凝聚素。
