Kontani H, Koshiura R
Jpn J Pharmacol. 1981 Dec;31(6):967-74. doi: 10.1254/jjp.31.967.
Afferent activities from receptors in the isolated lung of the bullfrog were recorded. Volley of afferent discharges synchronized with inflation of the lung and spontaneous discharges in the resting expiratory position were observed. When the solution of pH 9.5 was perfused in the pulmonary circulation, the rate of afferent discharges was slightly increased. When the pH was decreased to 5.5, the perfusion rate decreased and the rate of discharges was slightly increased initially, then markedly decreased. The amount of perfusion solution from the pulmonary vein per min and the firing frequency were almost restored to pretreatment level after the lung was perfused with normal Ringer's solution. When the temperature of the solution was from 20 degrees C to 10 degrees C, the rate of discharges was decreased. Epinephrine (1 x 10(-4) M) and 4-aminopyridine (1 x 10(-4) M and 1 x 10(-3) M) produced an increase in the rate of discharges in proportion to decrease in the perfusion rate. Histamine (1 x 10(-4) M) did not influence the firing frequency and the amount of perfusion solution from the vein per min. This method enables recording of the effect of drugs on the pulmonary afferent activities.
记录了牛蛙离体肺中感受器的传入活动。观察到传入放电的群峰与肺的充气同步，且在静息呼气位置有自发放电。当在肺循环中灌注pH 9.5的溶液时，传入放电频率略有增加。当pH降至5.5时，灌注速率降低，放电频率最初略有增加，然后显著降低。用正常林格氏液灌注肺后，每分钟肺静脉灌注液量和放电频率几乎恢复到预处理水平。当溶液温度从20℃降至10℃时，放电频率降低。肾上腺素（1×10⁻⁴ M）和4-氨基吡啶（1×10⁻⁴ M和1×10⁻³ M）使放电频率随灌注速率的降低而成比例增加。组胺（1×10⁻⁴ M）不影响放电频率和每分钟静脉灌注液量。该方法能够记录药物对肺传入活动的影响。
