Volgin A Iu, Pevnitskiĭ L A
Biull Eksp Biol Med. 1982 Jan;93(1):45-7.
A study was made of changes in the detectability of HLA-A, HLA-B and HLA-DR histocompatibility antigens on subpopulations of immunocompetent cells under the effect of mitogens and corticosteroids. HLA-antigens were identified by the lymphocytotoxic test. It was found that cell cultivation and mitogen stimulation lead to the fact that distinct reaction with the cells is produced by a greater number of HLA-antisera, while the specificity of direct and cross reactions remains unchanged. The main contribution to changes in the detectability of HLA-antigens is made by T cells. The exposure of the cells to dexamethasone in a dose producing no cytotoxic effect drastically lowered cell proliferation under the effect of PHA and deteriorated the detectability of HLA-A and HLA-B antigens. As regards HLA-DR antigens, it was disclosed that PM-stimulation of B cells considerably decreased the expression of their antigens, whereas PHA-stimulation of T cells led to the detection of HLA-DR antigens on these cells.
研究了有丝分裂原和皮质类固醇作用下免疫活性细胞亚群上HLA - A、HLA - B和HLA - DR组织相容性抗原可检测性的变化。通过淋巴细胞毒性试验鉴定HLA抗原。发现细胞培养和有丝分裂原刺激导致更多数量的HLA抗血清与细胞产生明显反应，而直接反应和交叉反应的特异性保持不变。T细胞对HLA抗原可检测性的变化起主要作用。细胞暴露于无细胞毒性作用剂量的地塞米松下，在PHA作用下细胞增殖大幅降低，且HLA - A和HLA - B抗原的可检测性变差。关于HLA - DR抗原，发现B细胞的PM刺激显著降低其抗原表达，而T细胞的PHA刺激导致在这些细胞上检测到HLA - DR抗原。
