一种用于分析针对人类组织相容性抗原的非补体结合单克隆抗体的抗球蛋白微细胞毒性试验。

An antiglobulin microcytotoxicity assay to analyze non-complement fixing monoclonal antibodies to human histocompatibility antigens.

作者信息

Pellegrino M, Russo C, Ng A K, Pellegrino A G, Ferrone S

出版信息

J Immunol Methods. 1982 Mar 12;49(2):119-28. doi: 10.1016/0022-1759(82)90270-8.

Abstract

An antiglobulin microcytotoxicity assay has been used to analyze non-complement fixing monoclonal antibodies to human histocompatibility antigens. The assay utilizes methodology similar to that of the widely used microcytotoxicity assay for HLA typing, requires low numbers of target cells and is suitable to test large numbers of samples. The sensitivity of the assay is influenced by the anti-mouse Ig antiserum used, by the sequence of addition of the various reagents and by the incubation time. The assay is suitable to screen supernatants of clones derived from hybridization experiments and to characterize the serological specificity of anti-HLA monoclonal antibodies.

摘要

抗球蛋白微细胞毒性试验已用于分析针对人类组织相容性抗原的非补体结合单克隆抗体。该试验采用的方法类似于广泛用于HLA分型的微细胞毒性试验，所需靶细胞数量少，适用于检测大量样本。该试验的灵敏度受所用抗小鼠Ig抗血清、各种试剂添加顺序以及孵育时间的影响。该试验适用于筛选杂交实验衍生克隆的上清液，并鉴定抗HLA单克隆抗体的血清学特异性。