Blank I H, Cohen J H, Anderson R R, Jaenicke K F, Parrish J A
J Invest Dermatol. 1982 May;78(5):381-5. doi: 10.1111/1523-1747.ep12507498.
A method is described for measuring the entrance into excised skin of ultraviolet radiation absorbing chemicals (UVRACs) following their application to the cutaneous surface in volatile, partially volatile or nonvolatile vehicles. Also a method is presented for observing changes in optical density (OD) of a sheet of stratum corneum subsequent to the application of an UVRAC and then washing it from the surface. Using these methods, p-aminobenzoic acid (PABA) and 2-ethylhexyl p-dimethyl aminobenzoate (O-PABA) have been studied. In the presence or absence of the nonvolatile vehicle, isopropyl myristate (IM), significant amounts of PABA enter the skin but almost all of the O-PABA remains on the surface. Nevertheless subsequently PABA is more easily removed by water than is O-PABA. When either UVRAC is applied to excised stratum corneum, the OD of the tissue increases immediately; only with PABA is there a further increase as it enters the skin. In vivo, delayed erythemal responses to 280-400 nm radiation of persons to whom the UVRACs are applied correlate well with the observations made on excised skin.
本文描述了一种测量方法，用于测定紫外线吸收化学物质（UVRACs）在以挥发性、部分挥发性或非挥发性载体涂覆于皮肤表面后进入离体皮肤的情况。同时还介绍了一种观察方法，用于观察在涂覆UVRAC并随后从表面冲洗掉之后角质层薄片的光密度（OD）变化。使用这些方法，对对氨基苯甲酸（PABA）和对二甲基氨基苯甲酸2-乙基己酯（O-PABA）进行了研究。在存在或不存在非挥发性载体肉豆蔻酸异丙酯（IM）的情况下，大量的PABA进入皮肤，但几乎所有的O-PABA都留在表面。然而，随后PABA比O-PABA更容易被水去除。当将任一种UVRAC涂覆于离体角质层时，组织的OD立即增加；只有PABA在进入皮肤时会有进一步增加。在体内，应用UVRAC的人对280 - 400nm辐射的延迟红斑反应与在离体皮肤上的观察结果密切相关。
