对聚（L-谷氨酸60-丙氨酸30-酪氨酸10）具有特异性的单克隆T细胞抑制因子的纯化与鉴定
Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10).
作者信息
Krupen K, Araneo B A, Brink L, Kapp J A, Stein S, Wieder K J, Webb D R
出版信息
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1254-8. doi: 10.1073/pnas.79.4.1254.
A monoclonal T-cell-derived suppressor factor specific for the terpolymer poly(LGlu60LAla30LTyr10) produced by the T-cell hybridoma 258 C4.4, was purified to homogeneity. This was accomplished by fractionation of the culture medium by using a combination of affinity chromatography and reverse-phase and ion-exchange high-performance liquid chromatography. The purified factor is composed of a single Mr 24,000 polypeptide chain, and the homogeneous protein maintains the ability to suppress antibody and T-cell proliferative responses to poly(LGlu60LAla30LTyr10) specifically. The specific activity of pure suppressor factor is calculated to be 8 X 10(7) units/micrograms.
由T细胞杂交瘤258 C4.4产生的、对三元共聚物聚（L-谷氨酸60-丙氨酸30-酪氨酸10）具有特异性的单克隆T细胞衍生抑制因子被纯化至同质。这是通过使用亲和色谱、反相和离子交换高效液相色谱相结合的方法对培养基进行分级分离来实现的。纯化后的因子由一条单一的分子量为24,000的多肽链组成，且该同质蛋白保持了特异性抑制对聚（L-谷氨酸60-丙氨酸30-酪氨酸10）的抗体和T细胞增殖反应的能力。纯抑制因子的比活性经计算为8×10⁷单位/微克。
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