登革病毒诱导的抑制性细胞因子的两条多肽链与其从巨噬细胞分离出的受体的结合。
Binding of the two polypeptide chains of dengue virus-induced suppressor cytokine to its receptor isolated from macrophages.
作者信息
Mukherjee R, Chaturvedi P, Chaturvedi U C
机构信息
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
出版信息
Int J Exp Pathol. 1993 Jun;74(3):259-66.
Dengue type 2 virus (DV)-induced suppressor cytokine (SF) is composed of two polypeptide chains (alpha and beta) and has a receptor on macrophages (M phi), which has been purified. The present study was undertaken to study the binding of the two polypeptide chains of SF to M phi and its purified receptor (SF-R) protein. It was observed that both alpha and beta-chains of SF, purified by high performance liquid chromatography (HPLC), bound to M phi cell, but only alpha-chain bound to SF-R protein. Only H-2A positive M phi bound SF. Anti-H-2Ak mAb blocked the binding of SF to M phi but had no effect on binding of SF to SF-R. Both anti-SF-antiserum and anti-SF-R-antiserum blocked the binding of SF to M phi. The binding of the beta-chain of SF to M phi was blocked by anti-H-2Ak mAb while the anti-SF-R-antiserum blocked the binding of alpha-chain on M phi. Thus, the alpha-chain of SF binds to SF-R and the beta-chain binds to H-2A determinants on M phi.
登革热2型病毒（DV）诱导的抑制性细胞因子（SF）由两条多肽链（α链和β链）组成，在巨噬细胞（M phi）上有一个已被纯化的受体。本研究旨在研究SF的两条多肽链与M phi及其纯化受体（SF-R）蛋白的结合情况。观察到通过高效液相色谱（HPLC）纯化的SF的α链和β链均与M phi细胞结合，但只有α链与SF-R蛋白结合。只有H-2A阳性的M phi能结合SF。抗H-2Ak单克隆抗体阻断了SF与M phi的结合，但对SF与SF-R的结合没有影响。抗SF抗血清和抗SF-R抗血清均阻断了SF与M phi的结合。抗H-2Ak单克隆抗体阻断了SF的β链与M phi的结合，而抗SF-R抗血清阻断了α链与M phi的结合。因此，SF的α链与SF-R结合，β链与M phi上的H-2A决定簇结合。
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