采用高效液相色谱-电化学检测法同时测定5-羟色氨酸和L-二羟基苯丙氨酸。测定离散脑区中血清素和儿茶酚胺的周转率。
Simultaneous measurement of 5-hydroxytryptophan and L-dihydroxyphenylalanine by high-performance liquid chromatography with electrochemical detection. Measurement of serotonin and catecholamine turnover in discrete brain regions.
作者信息
Shum A, Sole M J, Van Loon G R
出版信息
J Chromatogr. 1982 Mar 12;228:123-30. doi: 10.1016/s0378-4347(00)80425-9.
PMID:6978886
Abstract
A sensitive method, using reversed-phase high-performance liquid chromatography with electrochemical detection, which allows the measurement of both 5-hydroxytryptophan and L-dihydroxyphenylalanine is described. This method, used in conjunction with the administration in vivo of an L-aromatic amino acid decarboxylase inhibitor, allows the simultaneous measurement of serotonin and catecholamine synthesis rates in small discrete brain regions.
摘要
描述了一种灵敏的方法，该方法采用反相高效液相色谱法并结合电化学检测，可同时测定5-羟色氨酸和L-二羟基苯丙氨酸。此方法与体内给予L-芳香族氨基酸脱羧酶抑制剂相结合，能够同时测定小的离散脑区中5-羟色胺和儿茶酚胺的合成速率。
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