Julius M H, von Boehmer H, Sidman C L
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1989-93. doi: 10.1073/pnas.79.6.1989.
Cellular interactions involved in the T cell-dependent activation of B cells were analyzed by using lines and clones of helper T cells specific for determinants expressed on the B cell surface. Activation of male antigen-, M locus-, and H-2-specific T cells was shown to support polyclonal Ig production by a population of B cells that did not require T-cell-B-cell interaction for induction/amplification. However, these T cells alone did not activate gradient-purified small (resting) B cells. The activation of small B cells was shown to require not only a signal derived through an antigen-specific T-helper cell-B cell interaction but in addition a second signal that could be provided by anti-Ig antibodies.
通过使用针对B细胞表面表达的决定簇具有特异性的辅助性T细胞系和克隆，对参与B细胞T细胞依赖性激活的细胞间相互作用进行了分析。结果显示，雄性抗原特异性、M位点特异性和H-2特异性T细胞的激活能够支持一群B细胞的多克隆Ig产生，这群B细胞在诱导/扩增过程中不需要T细胞与B细胞的相互作用。然而，仅这些T细胞并不能激活梯度纯化的小（静止）B细胞。小B细胞的激活不仅需要通过抗原特异性辅助性T细胞与B细胞相互作用产生的信号，还需要抗Ig抗体提供的第二种信号。
