脉冲梯度31P核磁共振技术在蛙肌中的应用，用于测量细胞内磷化合物的扩散速率。
Application of pulsed-gradient 31P NMR on frog muscle to measure the diffusion rates of phosphorus compounds in cells.
作者信息
Yoshizaki K, Seo Y, Nishikawa H, Morimoto T
出版信息
Biophys J. 1982 May;38(2):209-11. doi: 10.1016/S0006-3495(82)84549-9.
Abstract
Pulsed-gradient 31P NMR was used to measure the diffusion rates of phosphorus compounds in aqueous solution and in living muscles. The diffusion rates of creatine phosphate and inorganic phosphate in intact frog muscle cells were reduced by a factor of approximately 2 from those in aqueous solution, which suggests that the apparent intracellular viscosity is approximately 2 times larger than in aqueous solution.
摘要
脉冲梯度31P核磁共振被用于测量磷化合物在水溶液和活体肌肉中的扩散速率。在完整的青蛙肌肉细胞中，磷酸肌酸和无机磷酸的扩散速率比在水溶液中的扩散速率降低了约2倍，这表明细胞内的表观粘度大约是水溶液中的2倍。
相似文献
1
Application of pulsed-gradient 31P NMR on frog muscle to measure the diffusion rates of phosphorus compounds in cells.脉冲梯度31P核磁共振技术在蛙肌中的应用，用于测量细胞内磷化合物的扩散速率。
Biophys J. 1982 May;38(2):209-11. doi: 10.1016/S0006-3495(82)84549-9.
2
Phosphate metabolism during muscular contraction in starved frogs (Rana catesbeiana).
Comp Biochem Physiol Comp Physiol. 1993 Dec;106(4):725-9.
3
High-energy phosphate turnover in muscle contraction: time-resolved 31P NMR studies.
Prog Clin Biol Res. 1989;315:185-95.
4
Regulation of glycogenolysis in contracting frog skeletal muscle studied by 31P nuclear magnetic resonance.
Prog Clin Biol Res. 1989;315:149-57.
5
31P-NMR study on intracellular energy transport in muscle.肌肉细胞内能量转运的31P核磁共振研究。
Prog Clin Biol Res. 1989;315:177-84.
6
In vivo 31P-NMR studies on aerobic recovery of frog muscle following tetanus.关于破伤风后青蛙肌肉有氧恢复的体内31P-核磁共振研究。
Jpn J Physiol. 1984;34(5):927-31. doi: 10.2170/jjphysiol.34.927.
7
Nuclear magnetic resonance studies on the mechanism of regulation of glycogenolysis in contracting skeletal muscle.
Biomed Biochim Acta. 1989;48(5-6):S335-40.
8
Diffusivities of creatine phosphate and ATP in an aqueous solution studied by pulsed field gradient 31P NMR.
Jpn J Physiol. 1987;37(5):923-8. doi: 10.2170/jjphysiol.37.923.
9
31P-NMR study of the regulation of glycogenolysis in living skeletal muscle.
Biochim Biophys Acta. 1987 Nov 12;931(2):170-4. doi: 10.1016/0167-4889(87)90203-5.
10
NMR monitoring of the anaerobic metabolism of frog muscle at rest.核磁共振监测静息状态下青蛙肌肉的无氧代谢。
Physiol Chem Phys Med NMR. 1990;22(4):233-40.
引用本文的文献
1
High-energy phosphate transfer in human muscle: diffusion of phosphocreatine.人体肌肉高能磷酸转移：磷酸肌酸的扩散。
Am J Physiol Cell Physiol. 2011 Jul;301(1):C234-41. doi: 10.1152/ajpcell.00500.2010. Epub 2011 Mar 2.
2
Molecules in motion: influences of diffusion on metabolic structure and function in skeletal muscle.运动中的分子：扩散对骨骼肌代谢结构和功能的影响。
J Exp Biol. 2011 Jan 15;214(Pt 2):263-74. doi: 10.1242/jeb.047985.
3
Philosophical basis and some historical aspects of systems biology: from Hegel to Noble - applications for bioenergetic research.系统生物学的哲学基础及部分历史：从黑格尔到诺贝尔——生物能量学研究的应用。
Int J Mol Sci. 2009 Mar;10(3):1161-92. doi: 10.3390/ijms10031161. Epub 2009 Mar 13.
4
On the origin of intracellular compartmentation and organized metabolic systems.论细胞内区室化和有组织的代谢系统的起源。
Mol Cell Biochem. 2004 Jan-Feb;256-257(1-2):5-12. doi: 10.1023/b:mcbi.0000009855.14648.2c.
5
Protein diffusion in living skeletal muscle fibers: dependence on protein size, fiber type, and contraction.蛋白质在活体骨骼肌纤维中的扩散：对蛋白质大小、纤维类型和收缩的依赖性。
Biophys J. 2000 Oct;79(4):2084-94. doi: 10.1016/S0006-3495(00)76456-3.
6
Depletion of phosphate in active muscle fibers probes actomyosin states within the powerstroke.活跃肌纤维中磷酸盐的耗尽探究了动力冲程内的肌动球蛋白状态。
Biophys J. 1998 Jan;74(1):369-80. doi: 10.1016/S0006-3495(98)77794-X.
7
Increase in ATP consumption during shortening in skinned fibres from rabbit psoas muscle: effects of inorganic phosphate.兔腰大肌去皮肤肌纤维收缩时ATP消耗的增加：无机磷酸盐的作用。
J Physiol. 1996 Oct 1;496 ( Pt 1)(Pt 1):1-12. doi: 10.1113/jphysiol.1996.sp021660.
8
Skeletal muscle: a paradigm for testing principles of bioenergetics.骨骼肌：用于测试生物能量学原理的范例。
J Bioenerg Biomembr. 1995 Dec;27(6):555-69. doi: 10.1007/BF02111654.
9
Influence of inorganic phosphate and pH on ATP utilization in fast and slow skeletal muscle fibers.无机磷酸盐和pH值对快、慢骨骼肌纤维中ATP利用的影响。
Biophys J. 1995 Dec;69(6):2580-9. doi: 10.1016/S0006-3495(95)80129-3.
10
Influence of phosphate and pH on myofibrillar ATPase activity and force in skinned cardiac trabeculae from rat.磷酸盐和pH值对大鼠去表皮心脏小梁肌原纤维ATP酶活性及张力的影响。
J Physiol. 1994 May 1;476(3):501-16. doi: 10.1113/jphysiol.1994.sp020150.
本文引用的文献
1
Rate of intracellular diffusion as measured in barnacle muscle.
Science. 1969 Jun 6;164(3884):1178-9. doi: 10.1126/science.164.3884.1178.
2
Ionic mobility in muscle cells.肌肉细胞中的离子迁移率。
Science. 1969 Dec 5;166(3910):1297-8. doi: 10.1126/science.166.3910.1297.
3
Restricted diffusion in biophysical systems. Experiment.生物物理系统中的受限扩散。实验。
Biophys J. 1974 Mar;14(3):161-77. doi: 10.1016/s0006-3495(74)85904-7.
4
Nuclear magnetic resonance measurement of skeletal muscle: anisotrophy of the diffusion coefficient of the intracellular water.骨骼肌的核磁共振测量：细胞内水扩散系数的各向异性
Biophys J. 1976 Sep;16(9):1043-53. doi: 10.1016/S0006-3495(76)85754-2.
5
Self diffusion of water in frog muscle.水在蛙肌中的自扩散
Biophys J. 1979 Oct;28(1):107-16. doi: 10.1016/S0006-3495(79)85162-0.
6
Phosphorus nuclear magnetic resonance studies of phosphorus metabolites in frog muscle.青蛙肌肉中磷代谢物的磷核磁共振研究。
J Biochem. 1978 Jul;84(1):11-8. doi: 10.1093/oxfordjournals.jbchem.a132098.
Zotero 插件