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正常小鼠对非主要组织相容性复合体同种异体抗原的原发性体外细胞毒性T细胞反应。

Primary in vitro cytotoxic T cell response to non-major histocompatibility complex alloantigens in normal mice.

作者信息

Macphail S, Yron I, Stutman O

出版信息

J Exp Med. 1982 Aug 1;156(2):610-21. doi: 10.1084/jem.156.2.610.

DOI:10.1084/jem.156.2.610
PMID:6980257
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2186758/
Abstract

We have shown for the first time that it is possible to consistently generate a primary in vitro cytotoxic T cell (Tc) response to non-major histocompatibility complex alloantigens using responder cells from a normal mouse strain. This was achieved by carrying out, in the generating phase, a limiting dilution procedure in which it appears that suppressor cells that inhibit Tc activation or expansion are too dilute to manifest their effect. Moreover, the response was observed in mouse serum-(MS) as well as fetal calf serum- (FCS) supplemented media, an important finding in the light of the anomalous nonspecific effects induced by FCS. The cytotoxic response produced in MS-supplemented media was shown to be highly specific in both the generating and effector phases, whereas the responses in FCS had a strong nonspecific component.

摘要

我们首次证明，使用来自正常小鼠品系的应答细胞，有可能持续产生针对非主要组织相容性复合体同种异体抗原的原发性体外细胞毒性T细胞（Tc）应答。这是通过在生成阶段进行有限稀释程序实现的，在该程序中，抑制Tc激活或扩增的抑制细胞似乎过于稀释，无法发挥其作用。此外，在补充了小鼠血清（MS）以及胎牛血清（FCS）的培养基中均观察到了这种应答，鉴于FCS诱导的异常非特异性效应，这是一个重要发现。在补充MS的培养基中产生的细胞毒性应答在生成阶段和效应阶段均显示出高度特异性，而在FCS中的应答则具有很强的非特异性成分。

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