Cooper K M, Moore M
J Clin Lab Immunol. 1982 Apr;7(3):155-61.
Circulating immune complex (IC) levels in sera from 58 healthy controls and a total of 212 patients with various chronic lung diseases were measured using 3 assays. Two complement-dependent assays (C1q fluid phase and Raji) and a complement-independent assay (L1210) were employed. The 3 assays generally revealed similar patterns of reactivity when control and pathological groups were compared, with the L1210 assay invariably demonstrating the lowest incidence of positive values in each group. The most significant elevations in IC levels were exhibited in bronchiectasis and bronchogenic carcinoma. However, within these two groups correlations between IC assays performed on individual sera were poor. Significant but weak correlations (p less than 0.01) were only observed for C1q vs Raji in bronchiectasis and C1q vs L1210 in bronchogenic carcinoma. Complement-binding ICs were present to a similar extent in both conditions. However, non-complement binding ICs were found to be more common in bronchiectasis. The relative sensitivity of the tests and the contribution of interfering factors to the disparity between the assays is discussed. However, the interpretation favoured is that the lack of correlation is primarily a reflection of the intrinsic heterogeneity of immune complexes formed under similar and dissimilar pathological conditions.
使用3种检测方法对58名健康对照者和总共212名患有各种慢性肺部疾病的患者血清中的循环免疫复合物（IC）水平进行了检测。采用了两种依赖补体的检测方法（C1q液相法和Raji法）和一种不依赖补体的检测方法（L1210法）。当比较对照组和病理组时，这3种检测方法通常显示出相似的反应模式，L1210检测法在每组中阳性值的发生率始终最低。IC水平升高最显著的是支气管扩张症和支气管源性癌。然而，在这两组中，对个体血清进行的IC检测之间的相关性较差。仅在支气管扩张症中观察到C1q与Raji之间以及在支气管源性癌中观察到C1q与L1210之间存在显著但较弱的相关性（p小于0.01）。在这两种情况下，补体结合IC的存在程度相似。然而，发现非补体结合IC在支气管扩张症中更为常见。讨论了检测方法的相对敏感性以及干扰因素对检测方法之间差异的影响。然而，倾向的解释是，缺乏相关性主要反映了在相似和不同病理条件下形成的免疫复合物的内在异质性。
