小鼠T细胞特异性Ia抗原：单克隆抗体确定一种由I-A编码的T淋巴细胞结构。

Murine T cell-specific Ia antigens: monoclonal antibodies define an I-A-encoded T lymphocyte structure.

作者信息

Hayes C E, Hullett D A

出版信息

Proc Natl Acad Sci U S A. 1982 Jun;79(11):3594-8. doi: 10.1073/pnas.79.11.3594.

DOI:10.1073/pnas.79.11.3594

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346469/

Abstract

T lymphocytes express a unique I-A subregion-controlled surface molecule that is not expressed on B lymphocytes. We produced antisera and monoclonal antibodies recognizing this structure. Exhaustive B cell adsorption (A.TH X B10.HTT)F antiserum, produced against activated A.TL T cells, left antibodies that bind an I-Ak specificity on some B10.A(4R) T lymphocytes (11 +/- 2%, mean +/- SEM). Similarly, exhaustive B cell adsorption of (A/J X B10.MBR)F antiserum, produced against activated B10.A(5R) cells, left antibodies specific for an I-Ab determinant on B10.A(5R)T cells (17 +/- 2%). We fused A.TL-immune (A.TH X B10.HTT)F splenocytes with NS-1 myeloma cells and identified antibody-producing hybrid cells by a fluorescent enzyme-linked immunosorbent microassay described herein. Eight monoclonal antibodies were selected; these lyse 7--26% of peripheral T cells from I-Ak strains. Thymocytes and bone marrow cells do not express the I-Ak T cells determinant. Exhaustive B cell adsorption did not remove I-Ak T cell-specific monoclonal antibodies.

摘要

T淋巴细胞表达一种独特的、受I-A亚区控制的表面分子，而B淋巴细胞不表达这种分子。我们制备了识别这种结构的抗血清和单克隆抗体。用针对活化的A.TL T细胞产生的抗血清（A.TH×B10.HTT）F进行彻底的B细胞吸附后，仍有一些抗体能与某些B10.A(4R) T淋巴细胞上的I-Ak特异性结合（11±2%，平均值±标准误）。同样，用针对活化的B10.A(5R)细胞产生的抗血清（A/J×B10.MBR）F进行彻底的B细胞吸附后，仍有针对B10.A(5R) T细胞上I-Ab决定簇的特异性抗体（17±2%）。我们将A.TL免疫的（A.TH×B10.HTT）F脾细胞与NS-1骨髓瘤细胞融合，并通过本文所述的荧光酶联免疫吸附微测定法鉴定产生抗体的杂交细胞。筛选出8种单克隆抗体；这些抗体可裂解来自I-Ak品系的7%-26%的外周T细胞。胸腺细胞和骨髓细胞不表达I-Ak T细胞决定簇。彻底的B细胞吸附并未去除I-Ak T细胞特异性单克隆抗体。

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SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.体外成纤维细胞交配产生的杂种及其假定重组体的选择

Science. 1964 Aug 14;145(3633):709-10. doi: 10.1126/science.145.3633.709.

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Studies on egg white and its constituents by immunochemical techniques in gelified media: specific precipitation by double diffusion and immunoelectrophoretic analysis.凝胶化介质中蛋清及其成分的免疫化学技术研究：双向扩散特异性沉淀及免疫电泳分析

J Immunol. 1955 Nov;75(5):367-76.

4

Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.分泌针对小鼠H-2和Ia抗原的单克隆抗体的杂交瘤细胞系。

J Immunol. 1980 Feb;124(2):533-40.

5

Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies.I-J基因产物对免疫反应的调节。I. 抗I-J单克隆抗体的产生与特性分析

J Exp Med. 1981 Nov 1;154(5):1570-83. doi: 10.1084/jem.154.5.1570.

6

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J Exp Med. 1981 Nov 1;154(5):1290-304. doi: 10.1084/jem.154.5.1290.

7

Reconstitution of Ir genes, Ia antigens, and mixed lymphocyte reaction determinants by gene complementation.通过基因互补重建Ir基因、Ia抗原和混合淋巴细胞反应决定簇。

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8

Murine T cell Ia antigens: specificity Iat.46 maps in I-J to I-E chromosomal segment of H-2 complex.小鼠T细胞Ia抗原：特异性Iat.46在H-2复合体的I-J至I-E染色体区段定位。

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