Lohrisch I, Scherbaum I, Gruhn R, Ambrosius H, Haustein U F, Herrmann K
Acta Biol Med Ger. 1981;40(12):1767-73.
Three day cultures of human peripheral blood lymphocytes (PBL) contain significant numbers of lymphoblasts binding 125I-alpha 1-AT as well as those with membrane-associated alpha 1-AT detectable by immunofluorescence. Co-cultivation of PBL with T cell mitogens leads to the appearance of lymphoblasts with membrane-associated alpha 1-AT while B cell mitogens induce lymphoblasts with a binding capacity for 125I-alpha 1-AT. It could be shown that unstimulated lymphocytes neither have membrane-associated alpha 1-AT nor possess a binding capacity for it. The highest number of lymphoblasts with membrane-associated alpha 1-AT was found in Con A stimulated cultures as revealed by indirect immunofluorescence. By contrast, PHA or PPD stimulated cultures contained much less such cells of lower fluorescence intensity. Co-cultivation of PBL with the B cell mitogens LPS and rabbit-anti-human gammaglobulin-IgG-F(ab')2 induced lymphoblasts with a clear binding capacity for 125I-alpha 1-AT, while dextran-sulphate had no effect. A possible regulative role of alpha 1-AT in the immune response is discussed.
人外周血淋巴细胞（PBL）的三天培养物中含有大量结合125I-α1-抗胰蛋白酶的淋巴母细胞，以及通过免疫荧光可检测到的具有膜相关α1-抗胰蛋白酶的细胞。PBL与T细胞有丝分裂原共培养会导致出现具有膜相关α1-抗胰蛋白酶的淋巴母细胞，而B细胞有丝分裂原则诱导出对125I-α1-抗胰蛋白酶具有结合能力的淋巴母细胞。可以证明，未受刺激的淋巴细胞既没有膜相关α1-抗胰蛋白酶，也不具备对其的结合能力。通过间接免疫荧光发现，在Con A刺激的培养物中，具有膜相关α1-抗胰蛋白酶的淋巴母细胞数量最多。相比之下，PHA或PPD刺激的培养物中这类细胞数量少得多，荧光强度也较低。PBL与B细胞有丝分裂原LPS和兔抗人丙种球蛋白-IgG-F(ab')2共培养可诱导出对125I-α1-抗胰蛋白酶具有明显结合能力的淋巴母细胞，而硫酸葡聚糖则没有作用。文中讨论了α1-抗胰蛋白酶在免疫反应中可能的调节作用。
