Zieliński A, Oehling A
Allergol Immunopathol (Madr). 1982 Mar-Apr;10(2):115-20.
A procedure is described for the cytochemical detection of esterase alpha naphtyl acetate (ANAE) and beta glucuronidase (BG) activity in the circulating lymphocytes of man. The number of BG positive cells (TBG) as evaluated on the smears of Ficoll Urovision purified cells, correlated well with the number of T gamma (EA gamma rosette forming cells with ox red blood cells) in the same blood samples of a group of healthy persons and patients, with pollinosis, atrophic rhinitis and bacterial asthma. In healthy persons, the number of T gamma cells by rosette method was 12.5 +/- 0.5% whilst BG positive cells were 14.0 +/- 14%. The patients had an equal number of BG positive cells and T gamma too. The present study broadly confirms ANAE activity as a cytochemical marker for T mu cells. These results tested that T lymphocytes can be accurately enumerated by cytochemical evaluation of ANAE activity in human lymphocytes and suggested the usefulness of BG activity in the enumeration of T lymphocytes in man.
本文描述了一种用于细胞化学检测人循环淋巴细胞中酯酶α萘乙酸酯（ANAE）和β葡萄糖醛酸酶（BG）活性的方法。在Ficoll Urovision纯化细胞涂片上评估的BG阳性细胞数量（TBG），与一组健康人和患有花粉症、萎缩性鼻炎及细菌性哮喘的患者的相同血样中Tγ细胞（与氧化红细胞形成EAγ玫瑰花结的细胞）数量密切相关。在健康人中，通过玫瑰花结法检测的Tγ细胞数量为12.5±0.5%，而BG阳性细胞为14.0±14%。患者的BG阳性细胞数量与Tγ细胞数量也相等。本研究广泛证实了ANAE活性作为Tμ细胞的细胞化学标志物。这些结果表明，通过对人淋巴细胞中ANAE活性进行细胞化学评估可以准确计数T淋巴细胞，并提示了BG活性在人T淋巴细胞计数中的有用性。
