Hull M G, Monro P P, Gillmer M D
Br J Obstet Gynaecol. 1978 Sep;85(9):645-51. doi: 10.1111/j.1471-0528.1978.tb14941.x.
Plasma unconjugated oestriol (E3) concentrations were determined by radioimmunoassay in 10 normal subjects in late pregnancy, throughout a normal day and night, and after a 50 g oral glucose load. There was a circadian pattern in E3 concentration characterized by an abrupt increase of 15 per cent at night. There was no significant rhythm during the day or night, taken separately. However there was a 10 to 11 per cent reduction in E3 concentrations soon after some meals and after a glucose load, possibly due to expansion of the plasma volume. Excluding the effect of meals, the fluctuation of E3 concentrations in individuals (median of the coefficients of variation) was 11.5 per cent in the day. Overall, it was 13.2 per cent in the day, 12.1 per cent at night and 15.9 per cent over the whole period studied. Since the variation in plasma unconjugated E3 concentrations through 24 hours was no greater than random fluctuations or day-to-day variation there is no need to restrict the time of blood sampling in clinical practice.
采用放射免疫分析法测定了10名妊娠晚期正常受试者在正常昼夜及口服50克葡萄糖负荷后的血浆非结合雌三醇（E3）浓度。E3浓度呈现昼夜节律模式，其特征为夜间突然升高15%。白天或夜间单独来看，均无明显节律。然而，在进食某些食物后以及葡萄糖负荷后不久，E3浓度会降低10%至11%，这可能是由于血浆容量扩张所致。排除进食的影响，个体中E3浓度的波动（变异系数中位数）在白天为11.5%。总体而言，白天为13.2%，夜间为12.1%，在整个研究期间为15.9%。由于24小时内血浆非结合E3浓度的变化不大于随机波动或每日变化，因此在临床实践中无需限制采血时间。
