Sobti R C, Krishan A, Pfaffenberger C D
Mutat Res. 1982 Jul-Aug;102(1):89-102. doi: 10.1016/0165-1218(82)90149-5.
In the present study, cytotoxic, cytostatic and cytogenetic effects of a number of organophosphate pesticides on human lymphoid cells (LAZ-007) in culture have been examined. Cytotoxic effects were dose related and often led to extensive cell kill. The pronounced effects of various organophosphates on the cell-cycle traverse were shown in data based on the enumeration of M1 and M3 metaphases after incubation of cells with BrdU. In cells incubated with 20 micrograms/ml of the various chemicals, the number of M1 metaphases ranged from 6% (R-1303) to 18% (Azodrin) compared to no M1 metaphases in control cultures. The number of M3s in cultures treated with 20 micrograms/ml of the various chemicals tested varied from 0% (Phosdrin) to 7% (parathion) as compared to 17% in control cultures. 11 out of the 14 organophosphates tested, significantly increased the SCE frequency. Of the 9 chemicals tested after metabolic activation by liver microsomal S9 preparation, significant increases in SCE frequency were seen in diazinon-, dimethoate-, Dursban- and Phosdrin-treated cells.
在本研究中，已检测了多种有机磷农药对培养的人淋巴细胞（LAZ - 007）的细胞毒性、细胞生长抑制和细胞遗传学效应。细胞毒性效应与剂量相关，且常常导致大量细胞死亡。基于用溴脱氧尿苷（BrdU）孵育细胞后对M1和M3中期的计数数据，显示了各种有机磷对细胞周期进程的显著影响。在用20微克/毫升的各种化学物质孵育的细胞中，与对照培养物中无M1中期相比，M1中期的数量范围为6%（R - 1303）至18%（久效磷）。在用所测试的20微克/毫升的各种化学物质处理的培养物中，M3的数量从0%（磷胺）至7%（对硫磷）不等，而对照培养物中为17%。在测试的14种有机磷中，有11种显著增加了姐妹染色单体交换（SCE）频率。在用肝微粒体S9制备物进行代谢活化后测试的9种化学物质中，在二嗪农（地亚农）、乐果、毒死蜱和磷胺处理的细胞中观察到SCE频率显著增加。
