活细胞中的荧光素激发和发射偏振光谱：细胞周期中的变化。
Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle.
作者信息
Cercek L, Cercek B, Ockey C H
出版信息
Biophys J. 1978 Sep;23(3):395-405. doi: 10.1016/S0006-3495(78)85458-7.
Abstract
Changes in the fluorescein fluorescence emission and excitation polarization spectra in synchronized cultured S3 fibroblasts at G1, mid-S, and mitosis, as well as in human lymphocytes before and after stimulation with mitogens, were studied. In contrast to those measured in aqueous solutions the emission and excitation polarization spectra in living cells exhibit a wavelength dependence characteristic for the state of the cell cycle. Changes in the temperature and in the amount of intracellular water result in quantitative wavelength-independent changes in the polarization spectra. Possible mechanisms for the qualitative wavelength-dependent changes in the fluorescein emission and excitation polarization spectra during the cell cycle are discussed.
摘要
研究了同步培养的S3成纤维细胞在G1期、S期中期和有丝分裂期，以及人淋巴细胞在有丝分裂原刺激前后的荧光素荧光发射和激发偏振光谱的变化。与在水溶液中测得的光谱相比，活细胞中的发射光谱和激发偏振光谱呈现出与细胞周期状态相关的波长依赖性特征。温度和细胞内水量的变化会导致偏振光谱发生与波长无关的定量变化。文中讨论了细胞周期中荧光素发射光谱和激发偏振光谱发生定性波长依赖性变化的可能机制。
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本文引用的文献
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