Blakeslee D
J Natl Cancer Inst. 1979 Aug;63(2):325-9.
The fluorescence polarization of human lymphocytes undergoing fluorescein fluorochromasia was measured in a system in which antifluorescein IgG was used to quench the extracellular emission of fluorescein leaked from the cells. The polarization values obtained from control cells were similar to those obtained by other investigators, as was the decrease in polarization in response to exposure to phytohemagglutinin or concanavalin A. However, the changes in polarization resulting from mitogen stimulation depended on the amount of antibody used, which suggests an increased or altered permeability of the plasma membrane to fluorescein efflux rather than a cytoplasmic reorganization.
在一个使用抗荧光素IgG淬灭从细胞泄漏的荧光素细胞外发射的系统中，测量了经历荧光素荧光染色的人淋巴细胞的荧光偏振。从对照细胞获得的偏振值与其他研究者获得的值相似，对暴露于植物血凝素或伴刀豆球蛋白A的偏振降低情况也相似。然而，有丝分裂原刺激引起的偏振变化取决于所用抗体的量，这表明质膜对荧光素外流的通透性增加或改变，而不是细胞质重组。
