Halper J, Kabat E A, Osserman E F, Tonda P, Pernis B
J Clin Invest. 1982 Dec;70(6):1292-9. doi: 10.1172/jci110728.
Recently we have identified two monoclonal immunoglobulin M (IgM) proteins that bind Klebsiella polysaccharides. The lymphocytes of one of these patients (M.A.Y.) were available for study. A substantial proportion of the B lymphocytes isolated from this patient's peripheral blood also bound Klebsiella polysaccharides with a pattern of specificity identical to that of the monoclonal IgM, and reacted with an anti-idiotypic antiserum directed against this IgM. Stripping the surface immunoglobulin from these lymphocytes eliminated this reactivity. Although no plasma cells were detected in the freshly isolated peripheral blood lymphocytes of this patient, plasma cells binding Klebsiella polysaccharide appeared after 7 d of in vitro culture. This occurred regardless of whether the cultures were supplemented with autologous plasma, normal human plasma, or fetal calf serum. Pokeweed mitogen neither stimulated nor inhibited the in vitro differentiation of the monoclonal B lymphocytes into plasma cells. This differentiation was, however, abrogated by F(ab')2 fragments of anti-human IgM and by anti-idiotypic antibodies, as well as by the Klebsiella polysaccharide with which the monoclonal IgM reacted.
最近，我们鉴定出了两种能结合克雷伯菌多糖的单克隆免疫球蛋白M（IgM）蛋白。其中一名患者（M.A.Y.）的淋巴细胞可供研究。从该患者外周血中分离出的相当一部分B淋巴细胞也能结合克雷伯菌多糖，其特异性模式与单克隆IgM相同，并能与针对该IgM的抗独特型抗血清发生反应。去除这些淋巴细胞表面的免疫球蛋白后，这种反应性消失。尽管在该患者新鲜分离的外周血淋巴细胞中未检测到浆细胞，但在体外培养7天后出现了结合克雷伯菌多糖的浆细胞。无论培养物中添加的是自体血浆、正常人血浆还是胎牛血清，都会出现这种情况。商陆有丝分裂原既不刺激也不抑制单克隆B淋巴细胞向浆细胞的体外分化。然而，抗人IgM的F(ab')2片段、抗独特型抗体以及单克隆IgM所反应的克雷伯菌多糖可消除这种分化。
