一种单克隆T细胞替代活性可直接作用于B细胞以增强克隆扩增。
A monoclonal T cell-replacing activity can act directly on B cells to enhance clonal expansion.
作者信息
Wetzel G D, Swain S L, Dutton R W
出版信息
J Exp Med. 1982 Jul 1;156(1):306-11. doi: 10.1084/jem.156.1.306.
Abstract
We have used a B cell cloning system in which the response of a single isolated B cell to lipopolysaccharide and dextran sulfide can be followed. We have shown that culture supernatants from the Dennert long-term alloreactive T cell line C.C3.11.75 increase the frequency of B cells stimulated to clonal expansion by mitogens. These culture supernatants are devoid of interleukin 1 and 2 but contain the T cell-replacing factor activity (DL)TRF. These experiments provide unequivocal proof that a T cell-derived factor or factors can act directly on a B lymphocyte in the absence of any other cell.
摘要
我们使用了一种B细胞克隆系统，通过该系统可以追踪单个分离的B细胞对脂多糖和硫化葡聚糖的反应。我们已经表明，来自Dennert长期同种异体反应性T细胞系C.C3.11.75的培养上清液可增加被有丝分裂原刺激而发生克隆扩增的B细胞频率。这些培养上清液不含白细胞介素1和2，但含有T细胞替代因子活性（DL）TRF。这些实验提供了明确的证据，证明一种或多种T细胞衍生因子可以在没有任何其他细胞的情况下直接作用于B淋巴细胞。
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