Cavaillon J M, Alouf J E
Immunol Lett. 1982;5(6):317-22. doi: 10.1016/0165-2478(82)90120-1.
Extracellular products have been purified from group A Streptococcus pyogenes culture supernatant fluids and their mitogenicities have been tested on rabbit and mouse lymphocytes. Two fractions were mitogenic: the kappa-fraction (pI = 4.8, mol. wt. = 30,000), a protein which was identified as the erythrogenic toxin, and the epsilon-fraction (pI = 10.3, mol. wt. = 17,000) a glycoprotein, both stimulated rabbit and CBA mouse spleen cells. The stimulation of rabbit thymocytes was weak unless macrophages or 2-mercaptoethanol were added. A third product, the gamma-fraction (a protein, pI = 4.2, mol. wt. = 72,000) was very weakly mitogenic and had the capacity to reduce the stimulation induced by a T-cell mitogen, such as Con A, but not by a B-cell mitogen such as Nocardia.
已从化脓性A组链球菌培养上清液中纯化出细胞外产物，并在兔和小鼠淋巴细胞上测试了它们的促有丝分裂活性。有两个组分具有促有丝分裂活性：κ组分（pI = 4.8，分子量 = 30,000），一种被鉴定为产红疹毒素的蛋白质，以及ε组分（pI = 10.3，分子量 = 17,000），一种糖蛋白，二者均能刺激兔和CBA小鼠的脾细胞。兔胸腺细胞的刺激作用较弱，除非添加巨噬细胞或2-巯基乙醇。第三种产物，γ组分（一种蛋白质，pI = 4.2，分子量 = 72,000）的促有丝分裂活性非常弱，并且有能力降低由T细胞促有丝分裂原（如刀豆球蛋白A）诱导的刺激，但不能降低由B细胞促有丝分裂原（如诺卡氏菌）诱导的刺激。
