Ni B, Brown I R
Department of Zoology, University of Toronto Scarborough Campus, West Hill, Ontario, Canada.
Neurochem Res. 1993 Feb;18(2):185-92. doi: 10.1007/BF01474683.
Reserpine evokes transsynaptic impulse activity by depleting catecholaminergic neurotransmitters in the rat brain. Previous studies suggest a relationship between catecholaminergic activity and calmodulin concentration. In this report we employ Northern blot analysis to examine the effect of a single subcutaneous injection of reserpine on levels of calmodulin mRNA species which are preferentially expressed in neurons of the rat brain. Regional differences in mRNA levels were also investigated by in situ hybridization and drug-induced changes were noted particularly in specific regions of the rat brain stem. The riboprobe used in the in situ hybridization study recognized a 4.0 kilobase neuronal calmodulin mRNA species (NGB1), which was derived from the rat CaM1 gene. A calmodulin radio-immunoassay was utilized to demonstrate a drug-induced increased in calmodulin protein levels in a region which included the brain stem.
利血平通过耗尽大鼠脑中的儿茶酚胺能神经递质来引发跨突触冲动活动。先前的研究表明儿茶酚胺能活性与钙调蛋白浓度之间存在关联。在本报告中，我们采用Northern印迹分析来检测单次皮下注射利血平对在大鼠脑神经元中优先表达的钙调蛋白mRNA种类水平的影响。还通过原位杂交研究了mRNA水平的区域差异，并特别注意到在大鼠脑干的特定区域中药物诱导的变化。原位杂交研究中使用的核糖探针识别出一种4.0千碱基的神经元钙调蛋白mRNA种类（NGB1），它源自大鼠CaM1基因。利用钙调蛋白放射免疫测定法来证明在包括脑干的一个区域中药物诱导的钙调蛋白蛋白质水平增加。
