Khodr G S, Siler-Khodr T M
Science. 1980 Jan 18;207(4428):315-7. doi: 10.1126/science.6985750.
The synthesis of a placental luteinizing hormone-releasing factor (pLRF), which is immunologically, physiochemically, and biologically indistinguishable from synthetic LRF, was demonstrated. The incorporation of 3H-labeled leucine by human placental tissue in vitro into pLRF was determined by purification on carboxymethyl-cellulose and specific immunoprecipitation of the 3H-labeled pLRF. The specific activity of the pLRF released into the medium increased 100-fold from day 1 to day 2 of culture and attained a concentration of 2.84 microcuries per microgram. These data indicate that the pLRF that was released initially was endogenous, whereas that released subsequently reflected synthesis.
已证实合成了一种胎盘促黄体生成素释放因子（pLRF），它在免疫学、物理化学和生物学性质上与合成的LRF无法区分。通过羧甲基纤维素纯化以及对3H标记的pLRF进行特异性免疫沉淀，测定了人胎盘组织在体外将3H标记的亮氨酸掺入pLRF的情况。释放到培养基中的pLRF的比活性在培养的第1天到第2天增加了100倍，达到每微克2.84微居里的浓度。这些数据表明，最初释放的pLRF是内源性的，而随后释放的则反映了合成情况。
