用于检测霍乱弧菌和大肠杆菌不耐热肠毒素及抗毒素的微量滴定板神经节苷脂酶联免疫吸附测定法
Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin.
作者信息
Sack D A, Huda S, Neogi P K, Daniel R R, Spira W M
出版信息
J Clin Microbiol. 1980 Jan;11(1):35-40. doi: 10.1128/jcm.11.1.35-40.1980.
Abstract
We have developed a microtiter enzyme-linked immunosorbent assay method for detecting the heat-labile enterotoxins of Vibrio cholerae and Escherichia coli using GM1 ganglioside as the base coat. This method compares favorably with a similar assay using anticholera toxin as the base coat, and with the Y1 adrenal cell assay. The assay should be useful in detecting enterotoxin production in E. coli and vibrios (including non-agglutinating Vibrio), in quantitating the toxin, and in determining binding properties of enterotoxins to ganglioside. The assay can also be used to quantitate antibodies which block the attachment of the toxin to the ganglioside.
摘要
我们已经开发出一种微量滴定酶联免疫吸附测定法，以GM1神经节苷脂为包被，用于检测霍乱弧菌和大肠杆菌的不耐热肠毒素。该方法与以抗霍乱毒素为包被的类似测定法以及Y1肾上腺细胞测定法相比具有优势。该测定法在检测大肠杆菌和弧菌（包括非凝集性弧菌）中的肠毒素产生、定量毒素以及确定肠毒素与神经节苷脂的结合特性方面应是有用的。该测定法还可用于定量阻断毒素与神经节苷脂结合的抗体。
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