Brill B M, Wasilauskas B L, Richardson S H
J Clin Microbiol. 1979 Jan;9(1):49-55. doi: 10.1128/jcm.9.1.49-55.1979.
Protein A-containing staphylococci coated with specific antiserum were tested for heat-labile enterotoxin of Escherichia coli. The immunological cross-reactivity of E. coli heat-labile enterotoxin with Vibrio cholerae toxin (choleragen) was the basis for sensitizing stabilized suspensions of the Cowan I strain of Staphylococcus aureus with anticholeragen. Unconcentrated culture supernatant fluid containing E. coli heat-labile enterotoxin produced macroscopic agglutination when mixed with sensitized staphylococci in capillary tubes. A total of 15 toxigenic and 61 nontoxigenic isolates were tested by the staphylococcal coagglutination technique in a coded fashion and found to be in agreement with previous results of the Chinese hamster ovary cell assay and the passive immune hemolysis test. The staphylococcal coagglutination technique is simple, relatively inexpensive to perform, and requires the immunoglobulin fraction of anticholeragen as the only specific reagent. The staphylococcal coagglutination technique appears to have potential for routine use in diagnostic microbiology laboratories.
用含有特异性抗血清包被的含蛋白A葡萄球菌检测大肠杆菌不耐热肠毒素。大肠杆菌不耐热肠毒素与霍乱弧菌毒素（霍乱原）的免疫交叉反应是用抗霍乱原致敏金黄色葡萄球菌考恩I菌株稳定悬液的基础。当含有大肠杆菌不耐热肠毒素的未浓缩培养上清液与毛细管中的致敏葡萄球菌混合时，会产生肉眼可见的凝集。采用葡萄球菌协同凝集技术以编码方式对总共15株产毒素菌株和61株不产毒素菌株进行了检测，结果与先前中国仓鼠卵巢细胞试验和被动免疫溶血试验的结果一致。葡萄球菌协同凝集技术操作简单、成本相对较低，并且仅需要抗霍乱原的免疫球蛋白部分作为唯一的特异性试剂。葡萄球菌协同凝集技术似乎有在诊断微生物学实验室常规使用的潜力。
