Gerber M A, Gray E D, Ferrieri P, Kaplan E L
J Lab Clin Med. 1980 Feb;95(2):258-65.
An ELISA method for determining the streptococcal anti--DNase B titers in human sera is presented. The details of this technique, a method for converting A493 readings into titers, and a standardization procedure are discussed. Anti--DNase B titers of 20 sera determined by ELISA were compared with titers determined by the microtechnique. A correlation coefficient of 0.96 between the two methods was obtained. The reproducibility of the ELISA method was established by comparing titers obtained from two separate determinations on the 20 sera. Twenty-four pairs of acute and convalescent sera were assayed for anti--DNase B titers by both ELISA and microtechnique to compare the ability of the two techniques to identify significant titer rises. The ELISA was as specific and possibly more sensitive than the microtechnique in identifying significant anti--DNase B titer rises. This ELISA method is simple and rapid and has the potential for automation.
本文介绍了一种用于测定人血清中链球菌抗脱氧核糖核酸酶B滴度的酶联免疫吸附测定（ELISA）方法。文中讨论了该技术的细节、将A493读数转换为滴度的方法以及标准化程序。通过ELISA测定的20份血清的抗脱氧核糖核酸酶B滴度与通过微量技术测定的滴度进行了比较。两种方法之间的相关系数为0.96。通过比较对20份血清进行两次单独测定所得的滴度，确定了ELISA方法的可重复性。通过ELISA和微量技术对24对急性和恢复期血清进行抗脱氧核糖核酸酶B滴度检测，以比较两种技术识别显著滴度升高的能力。在识别显著的抗脱氧核糖核酸酶B滴度升高方面，ELISA与微量技术一样具有特异性，且可能更敏感。这种ELISA方法简单快速，具有自动化的潜力。
