通过酶联免疫吸附测定法检测抗3型肺炎球菌荚膜多糖的免疫球蛋白G和M抗体。
Measurement of immunoglobulin G and M antibodies to type 3 pneumococcal capsular polysaccharide by enzyme-linked immunosorbent assay.
作者信息
Carlson B A, Giebink G S, Spika J S, Gray E D
出版信息
J Clin Microbiol. 1982 Jul;16(1):63-9. doi: 10.1128/jcm.16.1.63-69.1982.
Abstract
An enzyme-linked immunosorbent assay was developed to measure immunoglobulin G (IgG) and IgM type 3 antipneumococcal capsular polysaccharide antibodies. The use of Fab2 fragments of rabbit antipneumococcal IgG antibody in the antibody-antigen sandwich increased the sensitivity for measuring IgM antibodies and decreased background activity in antigen-free cuvettes. This methodology detected type 3 IgM antibody responses in six of six subjects vaccinated with polyvalent pneumococcal vaccine and detected type 3 IgG antibody responses in three subjects. Results of the enzyme-linked immunosorbent assay and radioimmunoassay procedures were concordant, and postvaccination enzyme-linked immunosorbent assay IgM titers showed a stronger correlation with total radioimmunoassay antibody than did postvaccination ELISA IgG titers.
摘要
开发了一种酶联免疫吸附测定法来测量免疫球蛋白G（IgG）和IgM 3型抗肺炎球菌荚膜多糖抗体。在抗体-抗原夹心测定中使用兔抗肺炎球菌IgG抗体的Fab2片段可提高测量IgM抗体的灵敏度，并降低无抗原比色皿中的背景活性。该方法在接种多价肺炎球菌疫苗的6名受试者中检测到了6例3型IgM抗体反应，并在3名受试者中检测到了3型IgG抗体反应。酶联免疫吸附测定法和放射免疫测定法的结果一致，接种疫苗后的酶联免疫吸附测定法IgM滴度与总放射免疫测定抗体的相关性比接种疫苗后的酶联免疫吸附测定法IgG滴度更强。
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本文引用的文献
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Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测到的针对肺炎球菌多糖的免疫球蛋白G和M抗体。
Infect Immun. 1980 Feb;27(2):411-7. doi: 10.1128/iai.27.2.411-417.1980.
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Serum antibody and opsonic responses to vaccination with pneumococcal capsular polysaccharide in normal and splenectomized children.正常儿童和脾切除儿童对肺炎球菌荚膜多糖疫苗接种的血清抗体及调理素反应。
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