Bergholtz B O, Thorsby E
Scand J Immunol. 1978;8(1):63-73. doi: 10.1111/j.1365-3083.1978.tb00496.x.
The response of T cells from sensitized individuals to low doses of PPD in vitro is macrophage-dependent. By testing different allogeneic combinations of macrophages and T lymphocytes, it was found that an optimal response required that antigen be presented by macrophages sharing at least one of the HLA-D determinants of the T cell donor. Antisera recognizing HLA-A, -B or -DR antigens were found to be able to inhibit this proliferative response. The anti-HLA-DR antisera were found to exert their inhibitory effect only when directed towards an antigen shared by the donors of the T lymphocytes and the macrophages. Anti-HLA-A and -B sera, however, had an inhibitory effect when reactive with the responding T lymphocytes, irrespective of their reactivity with the cooperating macrophages. It is concluded that an optimal secondary response of in-vivo-immunized T lymphocytes to PPD requires the combined recognition of the antigen and 'self' membrane structures encoded by the HLA-D locus.
致敏个体的T细胞在体外对低剂量结核菌素纯蛋白衍生物（PPD）的反应是巨噬细胞依赖性的。通过检测巨噬细胞和T淋巴细胞的不同同种异体组合，发现最佳反应要求抗原由至少共享T细胞供体的一个HLA - D决定簇的巨噬细胞呈递。发现识别HLA - A、- B或 - DR抗原的抗血清能够抑制这种增殖反应。发现抗HLA - DR抗血清仅在针对T淋巴细胞和巨噬细胞供体共有的抗原时才发挥其抑制作用。然而，抗HLA - A和 - B血清在与反应性T淋巴细胞反应时具有抑制作用，而不论它们与协同巨噬细胞的反应性如何。得出的结论是，体内免疫的T淋巴细胞对PPD的最佳二次反应需要抗原和由HLA - D基因座编码的“自身”膜结构的联合识别。
