Issekutz A C, Movat K W, Movat H Z
Clin Exp Immunol. 1980 Sep;41(3):505-11.
The intradermal injection into rabbits of various phagocytosible materials is known to induce polymorphonuclear leucocyte infiltration, protein exudation and microvascular haemorrhage. The injection of rabbit plasma, in which complement is activated with zymosan, also induces polymorphonuclear leucocyte infiltration. We investigated the effect of this soluble, non-ingestible stimulus to polymorphonuclear leucocyte accumulation on vascular integrity. Vascular permeability, measured with 125I-human serum albumin, was markedly but only transiently increased 1 hr after zymosan-activated plasma injection. In contrast, haemorrhage or red blood cell extravasation, measured with 59Fe-labelled red cells, developed only 4 hr after zymosan-activated plasma injection and continued for at least 10 hr. A direct correlation was found between the number of infiltrating polymorphonuclear leucocytes as measured with 51Cr-labelled leucocytes, and the degree of haemorrhage. Histological sections confirmed these changes. Plasma that was heat-inactivated (56 degrees C, 30 min) or made functionally C5-deficient prior to zymosan activation, failed to induce haemorrhage and caused no, or only mild , leucocyte infiltration. It is postulated that the C5ades arg in zymosan-activated plasma not only induces polymorphonuclear leucocyte infiltration but also, directly or indirectly, causes microvascular damage.
已知向兔子皮内注射各种可被吞噬的物质会诱导多形核白细胞浸润、蛋白质渗出和微血管出血。注射用酵母聚糖激活补体的兔血浆也会诱导多形核白细胞浸润。我们研究了这种可溶性、不可吞噬的刺激物对多形核白细胞聚集以及血管完整性的影响。用125I-人血清白蛋白测量，在注射酵母聚糖激活的血浆1小时后，血管通透性显著但只是短暂增加。相比之下，用59Fe标记的红细胞测量的出血或红细胞外渗，在注射酵母聚糖激活的血浆4小时后才出现，并持续至少10小时。用51Cr标记的白细胞测量的浸润多形核白细胞数量与出血程度之间存在直接相关性。组织学切片证实了这些变化。在酵母聚糖激活之前经热灭活（56℃，30分钟）或功能上缺乏C5的血浆，未能诱导出血，且未引起或仅引起轻度白细胞浸润。据推测，酵母聚糖激活的血浆中的C5ades arg不仅诱导多形核白细胞浸润，而且直接或间接导致微血管损伤。
