Tilghman S M, Curtis P J, Tiemeier D C, Leder P, Weissmann C
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1309-13. doi: 10.1073/pnas.75.3.1309.
Mouse beta-globin in encoded in a discontinuous structural gene interrupted by a 550-base pair intervening sequence of DNA. Correspondingly, the mature beta-globin mRNA appears to be synthesized via a 15S precursor, the length of which roughly equals the total length of the coding and intervening sequences of the beta-globin gene. Using the electron microscope to visualize hybrid structures formed between this gene and the purified 15S beta-globin mRNA precursor, we show that the intervening sequence is present within the larger precursor molecule. This finding suggests that the precursor mRNA is processed through the removal and rejoining of internal RNA sequences.
小鼠β-珠蛋白由一个不连续的结构基因编码，该基因被一段550个碱基对的DNA间隔序列中断。相应地，成熟的β-珠蛋白mRNA似乎是通过一个15S前体合成的，其长度大致等于β-珠蛋白基因编码序列和间隔序列的总长度。利用电子显微镜观察该基因与纯化的15Sβ-珠蛋白mRNA前体之间形成的杂交结构，我们发现间隔序列存在于较大的前体分子中。这一发现表明，前体mRNA是通过内部RNA序列的去除和重新连接进行加工的。
